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Literature summary for 4.6.1.16 extracted from

  • Randau, L.; Calvin, K.; Hall, M.; Yuan, J.; Podar, M.; Li, H.; Soll, D.
    The heteromeric Nanoarchaeum equitans splicing endonuclease cleaves noncanonical bulge-helix-bulge motifs of joined tRNA halves (2005), Proc. Natl. Acad. Sci. USA, 102, 17934-17939.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of genes NEQ205 and NEQ261 in Escherichia coli strain BL21(DE3), phylogenetic analysis Nanoarchaeum equitans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nanoarchaeum equitans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nanoarchaeum equitans the enzyme is required for tRNA maturation removing an intervening sequence element from the tRNA precursor ?
-
?

Organism

Organism UniProt Comment Textmining
Nanoarchaeum equitans
-
-
-

Reaction

Reaction Comment Organism Reaction ID
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus substrate recognition mechanism Nanoarchaeum equitans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is required for tRNA maturation removing an intervening sequence element from the tRNA precursor Nanoarchaeum equitans ?
-
?
additional information the enzyme cleaves noncanonical bulge-helix-bulge motifs of joined tRNA halves, e.g. of tRNAGlu, tRNAGln, and tRNALys, and removes an intervening sequence element from the tRNA precursor, cleavage pattern determination, overview Nanoarchaeum equitans ?
-
?

Subunits

Subunits Comment Organism
More heteromeric endonuclease type Nanoarchaeum equitans

Synonyms

Synonyms Comment Organism
splicing endonuclease
-
Nanoarchaeum equitans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
65
-
assay at Nanoarchaeum equitans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Nanoarchaeum equitans