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Literature summary for 4.6.1.16 extracted from
- Coevolution of tRNA intron motifs and tRNA endonuclease architecture in archaea (2005), Proc. Natl. Acad. Sci. USA, 102, 15418-15422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ARCFU, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Archaeoglobus fulgidus |
| gene METJA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Methanocaldococcus jannaschii |
| gene SULSO, DNA and amino acid sequence determination and analysis, coexpression of His-tagged SULSO alpha-subunit and untagged beta-subunit in Escherichia coli strain BL21(DE3) | Saccharolobus solfataricus |
| gene XENLA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Xenopus laevis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Xenopus laevis |  |
| Mg2+ | - |
Saccharolobus solfataricus | |
| Mg2+ | - |
Methanocaldococcus jannaschii | |
| Mg2+ | - |
Archaeoglobus fulgidus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
gene ARCFU | - |
| Methanocaldococcus jannaschii | - |
gene METJA | - |
| Saccharolobus solfataricus | - |
gene SULSO | - |
| Xenopus laevis | - |
gene XENLA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| copurification of recombinant His-tagged SULSO alpha-subunit and untagged beta-subunit from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharolobus solfataricus |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Xenopus laevis |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Methanocaldococcus jannaschii |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs and bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview | Saccharolobus solfataricus | ? | - |
? | |
| additional information | the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview | Xenopus laevis | ? | - |
? | |
| additional information | the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview | Archaeoglobus fulgidus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimer, alpha'2 enzyme architecture, structure overview | Archaeoglobus fulgidus |
| tetramer | heterotetramer, alpha2beta2 enzyme architecture, structure overview | Saccharolobus solfataricus |
| tetramer | homotetramer, alpha4 enzyme architecture, structure overview | Xenopus laevis |
| tetramer | homotetramer, alpha4 enzyme architecture, structure overview | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tRNA endonuclease | - |
Xenopus laevis |
| tRNA endonuclease | - |
Saccharolobus solfataricus |
| tRNA endonuclease | - |
Methanocaldococcus jannaschii |
| tRNA endonuclease | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Xenopus laevis |
| 65 | - |
assay at | Saccharolobus solfataricus |
| 65 | - |
assay at | Methanocaldococcus jannaschii |
| 65 | - |
assay at | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Xenopus laevis |
| 7.5 | - |
assay at | Saccharolobus solfataricus |
| 7.5 | - |
assay at | Methanocaldococcus jannaschii |
| 7.5 | - |
assay at | Archaeoglobus fulgidus |
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Release 2023.1 (January 2023)
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