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Literature summary for extracted from

  • Calvin, K.; Hall, M.D.; Xu, F.; Xue, S.; Li, H.
    Structural characterization of the catalytic subunit of a novel RNA splicing endonuclease (2005), J. Mol. Biol., 353, 952-960.
    View publication on PubMed


Cloned (Comment) Organism
genes endo1 and endo2, expression as His-tagged subunits forming the heterocomplex Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic subunit, X-ray diffraction structure determination and analysis at 3.1 A resolution Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
H136A site-directed mutagenesis of the catalytically essential residue results in an inactive mutant Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharolobus solfataricus the enzyme is responsible for recognition and excision of nuclear tRNA and all archeal introns ?


Organism UniProt Comment Textmining
Saccharolobus solfataricus
genes endo1 and endo2

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged subunits by nickel affinity chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is responsible for recognition and excision of nuclear tRNA and all archeal introns Saccharolobus solfataricus ?
additional information the enzyme of Sulfolobus solfataricus shows a broader substrate specificity Saccharolobus solfataricus ?


Subunits Comment Organism
dimer heterodimeric enzyme with subunit encoded by genes endo1 and endo2, analysis of the quarternary structure, structure of catalytic subunit, overview Saccharolobus solfataricus


Synonyms Comment Organism
RNA splicing endonuclease
Saccharolobus solfataricus