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Literature summary for 4.6.1.16 extracted from

  • Yoshinari, S.; Fujita, S.; Masui, R.; Kuramitsu, S.; Yokobori, S.; Kita, K.; Watanabe, Y.
    Functional reconstitution of a crenarchaeal splicing endonuclease in vitro (2005), Biochem. Biophys. Res. Commun., 334, 1254-1259.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
two distinct genes endA, ORFs ST0358 and STS047, DNA and amino acid sequence determination and analysis of two ORFs encoding the enzyme, one ORF lacks the putative catalytic amino acid residue, individual expression in Escherichia coli reveals that both are components of an enzyme complex Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
heteromeric enzyme complex composed of two components
-
Sulfurisphaera tokodaii 7
-
heteromeric enzyme complex composed of two components
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the heteromeric enzyme complex is composed of two components which are both required for catalytic activity, analysis of reaction products with synthetic tRNA substrate, overview Sulfurisphaera tokodaii ?
-
?
additional information the heteromeric enzyme complex is composed of two components which are both required for catalytic activity, analysis of reaction products with synthetic tRNA substrate, overview Sulfurisphaera tokodaii 7 ?
-
?

Subunits

Subunits Comment Organism
dimer heteromeric enzyme complex, both enzyme components are required for catalytic activity Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
splicing endonuclease
-
Sulfurisphaera tokodaii

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
70
-
assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Sulfurisphaera tokodaii