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Literature summary for 4.6.1.16 extracted from

  • Thompson, L.D.; Brandon, L.D.; Nieuwlandt, D.T.; Daniels, C.J.
    Transfer RNA intron processing in the halophilic archaebacteria (1989), Can. J. Microbiol., 35, 36-42.
    View publication on PubMed
Search for more literature for 4.6.1.16

Activating Compound

Activating Compound Comment Organism Structure
spermidine required Haloferax volcanii

Inhibitors

Inhibitors Comment Organism Structure
Mg2+
-
 Haloferax volcanii
monovalent cations at 50 mM Haloferax volcanii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane soluble and membrane bound Haloferax volcanii 16020
-
soluble soluble and membrane bound Haloferax volcanii
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA precursor Haloferax volcanii
-
 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA precursor
-
 Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
tRNA precursor substrate recognition at the exon-intron boundary region Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
tRNA precursor precise and accurate excision of the intron from halobacterial tRNATrp precursor Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
tRNA precursor does not require intact mature tRNA structure in the substrate Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Haloferax volcanii
7.5
-
 assay at Haloferax volcanii