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Literature summary for 4.6.1.16 extracted from

  • Peebles, C.L.; Gegenheimer, P.; Abelson, J.
    Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease (1983), Cell, 32, 525-536.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
nonionic detergents stimulate activity of the endonuclease in the membrane fraction Saccharomyces cerevisiae
spermidine or spermine required Saccharomyces cerevisiae
spermine or spermidine required, enhances extent and accuracy of cleavage, degree of stimulation varies with the pre-tRNA substrate Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Saccharomyces cerevisiae
Fe2+
-
Saccharomyces cerevisiae
Mg2+ at high concentrations Saccharomyces cerevisiae
Mn2+
-
Saccharomyces cerevisiae
monovalent cations at high concentrations Saccharomyces cerevisiae
Ni2+
-
Saccharomyces cerevisiae
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
Zn2+
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA precursor Saccharomyces cerevisiae
-
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor Saccharomyces cerevisiae M304
-
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae M304
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, strain M304 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA precursor
-
Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor specifically removing the intron Saccharomyces cerevisiae a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor
-
Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?
tRNA precursor specifically removing the intron Saccharomyces cerevisiae M304 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Saccharomyces cerevisiae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
37
-
rapid inactivation above Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 8 active in the range Saccharomyces cerevisiae