Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nonionic detergents | stimulate activity of the endonuclease in the membrane fraction | Saccharomyces cerevisiae | |
spermidine | or spermine required | Saccharomyces cerevisiae | |
spermine | or spermidine required, enhances extent and accuracy of cleavage, degree of stimulation varies with the pre-tRNA substrate | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Saccharomyces cerevisiae | |
Fe2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | at high concentrations | Saccharomyces cerevisiae | |
Mn2+ | - |
Saccharomyces cerevisiae | |
monovalent cations | at high concentrations | Saccharomyces cerevisiae | |
Ni2+ | - |
Saccharomyces cerevisiae | |
p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
Zn2+ | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Saccharomyces cerevisiae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor | Saccharomyces cerevisiae | - |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | Saccharomyces cerevisiae M304 | - |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae M304 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, strain M304 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor | - |
Saccharomyces cerevisiae | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini | Saccharomyces cerevisiae | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | specifically removing the intron | Saccharomyces cerevisiae | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | - |
Saccharomyces cerevisiae M304 | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini | Saccharomyces cerevisiae M304 | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? | |
tRNA precursor | specifically removing the intron | Saccharomyces cerevisiae M304 | a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
rapid inactivation above | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | active in the range | Saccharomyces cerevisiae |