Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
Y246A | the variant shows significant membrane binding defects | Bacillus thuringiensis |
Y246A/Y247A | the variant shows significant membrane binding defects | Bacillus thuringiensis |
Y247A | the variant shows significant membrane binding defects | Bacillus thuringiensis |
Y86A/Y88A | the mutations decrease membrane affinity for the enzyme | Bacillus thuringiensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus thuringiensis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34800 | - |
x * 34800, calculated from amino acid sequence | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol | Bacillus thuringiensis | - |
1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol | - |
? | |
1-phosphatidyl-1D-myo-inositol | Bacillus thuringiensis N168C | - |
1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | P08954 | - |
- |
Bacillus thuringiensis N168C | P08954 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and phenyl Sepharose column chromatography | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol | - |
Bacillus thuringiensis | 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol | - |
? | |
1-phosphatidyl-1D-myo-inositol | - |
Bacillus thuringiensis N168C | 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34800, calculated from amino acid sequence | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol-specific phospholipase C | - |
Bacillus thuringiensis |
PI-PLC | - |
Bacillus thuringiensis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a virulence factor | Bacillus thuringiensis |