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Literature summary for 4.6.1.13 extracted from
- Purification and properties of phosphatidylinositol-specific phospholipase C from Streptomyces antibioticus (1994), Biochim. Biophys. Acta, 1214, 221-228.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | - |
Streptomyces antibioticus |  |
| diacylglycerol | activates | Streptomyces antibioticus | |
| diethyl ether | strongly activates | Streptomyces antibioticus | |
| SDS | strongly activates | Streptomyces antibioticus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Streptomyces antibioticus | |
| EDTA | - |
Streptomyces antibioticus | |
| EGTA | - |
Streptomyces antibioticus | |
| Hg2+ | - |
Streptomyces antibioticus | |
| Zn2+ | - |
Streptomyces antibioticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Streptomyces antibioticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
x * 15000, alpha-peptide, + x * 23000, beta-peptide, SDS-PAGE | Streptomyces antibioticus |
| 23000 | - |
nondenaturing PAGE | Streptomyces antibioticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces antibioticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.1 | - |
- |
Streptomyces antibioticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol + H2O | specific for | Streptomyces antibioticus | diacylglycerol + myo-inositol 1,2-cyclic phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15000, alpha-peptide, + x * 23000, beta-peptide, SDS-PAGE | Streptomyces antibioticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces antibioticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 20°C: about 80% of maximal activity, 70°C: about 35% of maximal activity | Streptomyces antibioticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 8.0, 30 min, stable below | Streptomyces antibioticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces antibioticus |
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