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Literature summary for 4.6.1.12 extracted from

  • Liu, W.; Chen, M.; Yang, C.; Yang, Y.; Lan, X.; Liao, Z.
    A new 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase gene from Taxus media: Cloning, characterization and functional complementation (2009), J. Med. Plant Res., 3, 395-402.
No PubMed abstract available
Search for more literature for 4.6.1.12

Cloned(Commentary)

Cloned (Comment) Organism
a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS Taxus x media

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ active site including a Zn2+ ligand (Asp92-His94-His126) Taxus x media

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26100
-
 sequence analysis Taxus x media

Organism

Organism UniProt Comment Textmining
Taxus x media D3G6G3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bark tender bark, highest level Taxus x media
-
leaf highest level Taxus x media
-
root middle level Taxus x media
-
stem lowestlevel Taxus x media
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information active site including CDP-binding sites (Asp140Gly142 and Leu184Leu189Ser190Lys216Thr217Glu219). Owns a 60 amino-acid plastidial transit peptide at its N-terminus Taxus x media ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26100, calculated from sequence Taxus x media

Synonyms

Synonyms Comment Organism
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
-
 Taxus x media
MECPS
-
 Taxus x media

pI Value

Organism Comment pI Value Maximum pI Value
Taxus x media sequence analysis
-
 8.22

Expression

Organism Comment Expression
Taxus x media higher expression levels in tender barks and leaves than that in roots and stems up

General Information

General Information Comment Organism
physiological function is involved in the taxol precursor biosynthesis. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS can grow well, while Escherichia coli without exogenous plasmid or harboring either of the plasmids can not grow Taxus x media