Literature summary for 4.6.1.12 extracted from

Richard, S.B.; Ferrer, J.L.; Bowman, M.E.; Lillo, A.M.; Tetzlaff, C.N.; Cane, D.E.; Noel, J.P.
Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway (2002), J. Biol. Chem., 277, 8667-8672.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour diffusion method, X-ray crystal structures refined to 2.8 A resolution. The first structure contains a bound Mn2+ cation and the second structure contains CMP, 2-C-methyl-D-erythritol-2,4-cyclodiphosphate, and Mn2+	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the tetrahedrally arranged transition metal binding site, potentially occupied by Mn2+, sits at the base of the active site cleft. A phosphate oxygen of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate and the side chains of Asp8, His10, and His42 occupy the metal side chains of Asp8, His10, and His42 occupy the metal side coordination sphere	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P62617	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol	-	Escherichia coli	2C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP	-	?

Subunits

Subunits	Comment	Organism
trimer	homotrimeric quarternary structure built around a central hydrophobic cavity and three externally facing active sites	Escherichia coli

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Release 2023.1 (January 2023)