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Literature summary for 4.6.1.1 extracted from

  • Topal, H.; Fulcher, N.B.; Bitterman, J.; Salazar, E.; Buck, J.; Levin, L.R.; Cann, M.J.; Wolfgang, M.C.; Steegborn, C.
    Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa (2012), J. Mol. Biol., 416, 271-286.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli SG13009[pREP4] andM15[pREP4] cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 12% (w/v) polyethylene glycol 20000 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
A197T the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
E189R the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
E377G the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
F399H the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
F399I the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
I352T the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
L326P the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
R318W the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
R412H the mutant shows slightly increased activity compared to the wild type enzyme Pseudomonas aeruginosa
R456L the mutant shows strongly increased activity compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyestradiol significant inhibition at 0.1 mM Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Pseudomonas aeruginosa
-
 3',5'-cyclic AMP + diphosphate
-
 ?
ATP Pseudomonas aeruginosa ATCC 15692
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HZ23
-
-
Pseudomonas aeruginosa ATCC 15692 Q9HZ23
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superose 12 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Pseudomonas aeruginosa 3',5'-cyclic AMP + diphosphate
-
 ?
ATP
-
 Pseudomonas aeruginosa ATCC 15692 3',5'-cyclic AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
adenylyl cyclase
-
 Pseudomonas aeruginosa
CyaB
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function the enzyme is a key regulator of Pseudomonas aeruginosa virulence Pseudomonas aeruginosa