Cloned (Comment) | Organism |
---|---|
expression of the N-terminally His-tagged catalytic domain of CyaC | Arthrospira platensis |
expression of transmembrane isozymes in HEK293T cells, expression of GST-tagged soluble isozyme | Homo sapiens |
expression of transmembrane isozymes in HEK293T cells, expression of His-tagged soluble isozyme | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis | Arthrospira platensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,3,4,5,6,7,8,13,13,14,14-dodecachloro-1,4,4a,4b,5,8,8a,12b-octahydro-11-sulfo-1,4:5,8-dimethanotriphenylene-10-carboxylic acid | the compound defines an AC inhibitor scaffold with high affinity for human soluble isozyme and less inhibitory effect on mammalian transmembrane isozymes | Arthrospira platensis | |
1-(bromo(1-naphthyl)methyl)naphthalene | - |
Arthrospira platensis | |
17beta-estra-1(10),2,4-triene-2,3,17-triol | - |
Arthrospira platensis | |
2,3,6,23-tetrahydroxyurs-12-en-28-oic acid | - |
Arthrospira platensis | |
2,3-dibromo-1-(4-(hydroxy(oxido)amino)phenyl)-3-(4-quinolinyl)-1-propanone | - |
Arthrospira platensis | |
3,20-dioxopregn-4-en-21-yl 4-bromobenzenesulfonate | discriminates between soluble isozyme and transmembrane isozymes, and appears to simultaneously block the original binding pocket and a neighboring interaction site | Arthrospira platensis | |
4,5,6,7-tetrachloro-3,3-bis(6-hydroxy[1,1'-biphenyl]-3-yl)-2-benzofuran-1(3H)-one | - |
Arthrospira platensis | |
5'-benzyl-12'-hydroxy-2'-methyl-3',6',18-trioxoergotaman | - |
Arthrospira platensis | |
additional information | structure-based development of adenylyl cyclase inhibitors, compounds exploiting the catechol estrogen binding site can produce potent, isoform discriminating adenylyl cyclase inhibitors, inhibitory potency of compounds, overview | Arthrospira platensis | |
additional information | structure-based development of adenylyl cyclase inhibitors, compounds exploiting the catechol estrogen binding site can produce potent, isoform discriminating adenylyl cyclase inhibitors, inhibitory potency of compounds, overview | Homo sapiens | |
additional information | structure-based development of adenylyl cyclase inhibitors, compounds exploiting the catechol estrogen binding site can produce potent, isoform discriminating adenylyl cyclase inhibitors, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane isozymes | Rattus norvegicus | 16020 | - |
membrane | transmembrane isozymes | Homo sapiens | 16020 | - |
soluble | soluble isozyme | Rattus norvegicus | - |
- |
soluble | soluble isozyme | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | activates | Rattus norvegicus | |
bicarbonate | activates | Homo sapiens | |
Ca2+ | activates | Rattus norvegicus | |
Ca2+ | activates | Homo sapiens | |
Mg2+ | activates | Rattus norvegicus | |
Mg2+ | activates | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | Rattus norvegicus | - |
3',5'-cyclic-AMP + diphosphate | - |
? | |
ATP | Homo sapiens | - |
3',5'-cyclic-AMP + diphosphate | - |
? | |
ATP | Arthrospira platensis | - |
3',5'-cyclic-AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrospira platensis | O32393 | gene cyaC | - |
Homo sapiens | Q96PN6 | - |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble isozyme | Rattus norvegicus |
soluble isozyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Rattus norvegicus | 3',5'-cyclic-AMP + diphosphate | - |
? | |
ATP | - |
Homo sapiens | 3',5'-cyclic-AMP + diphosphate | - |
? | |
ATP | - |
Arthrospira platensis | 3',5'-cyclic-AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class III adenylate cyclase | - |
Arthrospira platensis |
cyaC | - |
Arthrospira platensis |
More | the enzyme belongs to the class III adenylyl cyclase superfamily | Arthrospira platensis |
sAC | - |
Rattus norvegicus |
sAC | - |
Homo sapiens |
soluble adenylate cyclase | - |
Rattus norvegicus |
soluble adenylate cyclase | - |
Homo sapiens |
tmAC | - |
Rattus norvegicus |
tmAC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
- |
Arthrospira platensis | 17beta-estra-1(10),2,4-triene-2,3,17-triol | |
0.005 | - |
- |
Arthrospira platensis | 1,2,3,4,5,6,7,8,13,13,14,14-dodecachloro-1,4,4a,4b,5,8,8a,12b-octahydro-11-sulfo-1,4:5,8-dimethanotriphenylene-10-carboxylic acid | |
0.015 | - |
- |
Arthrospira platensis | 3,20-dioxopregn-4-en-21-yl 4-bromobenzenesulfonate | |
0.07 | - |
about | Arthrospira platensis | 5'-benzyl-12'-hydroxy-2'-methyl-3',6',18-trioxoergotaman | |
0.1 | - |
about | Arthrospira platensis | 2,3-dibromo-1-(4-(hydroxy(oxido)amino)phenyl)-3-(4-quinolinyl)-1-propanone | |
0.1 | - |
about | Arthrospira platensis | 4,5,6,7-tetrachloro-3,3-bis(6-hydroxy[1,1'-biphenyl]-3-yl)-2-benzofuran-1(3H)-one | |
0.1 | - |
about | Arthrospira platensis | 2,3,6,23-tetrahydroxyurs-12-en-28-oic acid | |
0.2 | - |
about | Arthrospira platensis | 1-(bromo(1-naphthyl)methyl)naphthalene |