Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-CT | h5-HT6/rAC2 cell line highly responsive | Rattus norvegicus | |
5-HT | h5-HT6/rAC2 cell line highly responsive | Rattus norvegicus | |
lisuride | h5-HT6/rAC2 cell line highly responsive | Rattus norvegicus | |
phorbol 12-myristate 13-acetate | PKC-selective activator, stimulates AC2 | Rattus norvegicus | |
serotonin | AC2 stimulation followed by cAMP accumulation, no effect on AC5. AC2 co-transfected with Gs protein shows a synergistic effect on both baseline and agonist-stimulated cAMP accumulation. In the combined presence of Golf and AC2, the level of cAMP accumulation is enhanced in the agonist-stimulated cell line to levels equaling those of the AC2-expressing cell line only, with no effect on baseline activity. Similarly, when AC5 is co-expressed with Gs a synergistic effect with this pairing on both baseline and agonist-stimulated cAMP accumulation versus control can be observed. Because baseline activity is so high with the AC5/Gs pairing, the difference between stimulated and non-stimulated treatments is not significant. The AC5/Golf pairing fails to show any enhancement in cAMP accumulation upon serotonin stimulation, and no effect on baseline activity | Rattus norvegicus | |
sumatriptan | h5-HT6/rAC2 cell line highly responsive | Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
drug development | stable AC2-expressing CHODUKX cell line in which the generation of high expressing GPCR receptor/AC2 lines can retain their functional responsiveness and can provide pharmacological drug comparisons between the same host line for screening purposes and measurement of multiple cellular parameters | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
h5-HT6 pHTop CHODUKX-A2 cells transiently transfected with human Golf, Gs proteins and rat AC2, AC5 full-length isoforms | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | selective PKC inhibitor, abolishes cAMP accumulation by AC2 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
123000 | - |
AC2, Western immunoblotting | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | stable co-expression of AC2 with h5-HT6 receptor in CHODUKX cell line displays dose-dependent cAMP accumulation following agonist treatment | Rattus norvegicus | 3',5'-cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AC2 | - |
Rattus norvegicus |
AC5 | - |
Rattus norvegicus |
adenylyl cyclase 2 | - |
Rattus norvegicus |