Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.1 extracted from

  • Gao, X.; Sadana, R.; Dessauer, C.W.; Patel, T.B.
    Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits (2007), J. Biol. Chem., 282, 294-302.
    View publication on PubMed
Search for more literature for 4.6.1.1

Activating Compound

Activating Compound Comment Organism Structure
forskolin
-
 Rattus norvegicus
forskolin stimulates Gbetagamma subunits whereby enhancing the activity of enzymes Homo sapiens
forskolin stimulates the activity of soluble forms of ACV comprising their C1 and C2 domains, but Gbeta1gamma2 does not alter the ability of the agonist to regulate adenylate cyclase activity Canis lupus familiaris
Galphas
-
 Rattus norvegicus
Galphas stimulates Gbetagamma subunits whereby enhancing the activity of enzymes Homo sapiens
Galphas stimulates the activity of soluble forms of ACV comprising their C1 and C2 domains, but Gbeta1gamma2 does not alter the ability of the agonist to regulate adenylate cyclase activity Canis lupus familiaris
Gbeta1gamma2 increases the activation of ACV and ACVI by forskolin or Galphas. Gbetagamma subunits derived from sources other than Gi participate in mediating the full activation of ACVI by the beta-adrenergic receptor agonist, isoproterenol Homo sapiens
Gbetagamma conditionally stimulates ACV and ACVI Rattus norvegicus
Gbetagamma Gbetagamma conditionally stimulates ACVI, but does not stimulate ACV Canis lupus familiaris

Cloned(Commentary)

Cloned (Comment) Organism
ACV expressed in Sf9 cells Canis lupus familiaris
ACV fused with a GST tag and expressed in Escherichia coli, wild-type and mutant ACVIdeltaN enzymes expressed in Sf9 cells, COS-7 cells overexpressing ACVI, binding domain vector pGBKT7 together with pGADT7-beta1 or the activating vector pGADT7 together with pGBKT7-N-terminal region of ACV or ACVI co-transformed into AH109 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information mutant ACVIdeltaN, shows a much lower activity as compared with full-length ACVI, but its stimulated activity is about 4fold higher than the corresponding control activity, activities of the recombinant, engineered, soluble forms of ACV and ACVI, which lack the N termini, are not enhanced by Gbetagamma subunits. Deletion of residues 77-151, but not 1-76, in the N-terminal region of ACVI obliterates the ability of Gbetagamma subunits to conditionally stimulate the enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Galphai inhibits Galphas-stimulated activities of ACVI, Gbetagamma does not alter the ability of Galphai to inhibit the activities of ACVI Homo sapiens
additional information G protein coupled receptor kinase 2 by sequestering Gbetagamma subunits in vivo decreases isoproterenol-elicited increase in cAMP accumulation in cells expressing ACVI. Pertussis toxin treatment does not alter the ability of G protein coupled receptor kinase 2 to attenuate isoproterenol-stimulated ACVI activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ACV purified by gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Homo sapiens 3',5'-cAMP + diphosphate
-
 ?
ATP
-
 Rattus norvegicus 3',5'-cAMP + diphosphate
-
 ?
ATP
-
 Canis lupus familiaris 3',5'-cAMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ACV
-
 Homo sapiens
ACV
-
 Rattus norvegicus
ACV
-
 Canis lupus familiaris
ACVI
-
 Homo sapiens
ACVI
-
 Rattus norvegicus
ACVI
-
 Canis lupus familiaris
type VI adenylyl cyclase
-
 Homo sapiens