Literature summary for 4.6.1.1 extracted from

Beltran, C.; Vacquier, V.D.; Moy, G.; Chen, Y.; Buck, J.; Levin, L.R.; Darszon, A.
Particulate and soluble adenylyl cyclases participate in the sperm acrosome reaction (2007), Biochem. Biophys. Res. Commun., 358, 1128-1135.

Search for more literature for 4.6.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
bicarbonate	50 mM stimulate sAC activity 2fold	Strongylocentrotus purpuratus
forskolin	stimulates tmAC activity 1.6fold	Strongylocentrotus purpuratus
NaF	stimulates tmAC activity 2.9fold	Strongylocentrotus purpuratus

Application

Application	Comment	Organism
additional information	both sAC and tmACs participate in the sperm acrosome reaction and sperm motility	Strongylocentrotus purpuratus

Inhibitors

Inhibitors	Comment	Organism	Structure
2',5'-dideoxyadenosine	inhibits forskolin induced tmAC stimulation at 0.15 mM	Strongylocentrotus purpuratus
2-hydroxyestradiol	selective sAC inhibitor, inhibits the jelly induced acrosome reaction by 30% as well as the increase in cAMP levels by 42%	Strongylocentrotus purpuratus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	present in the head, acrosomal and mitochondrial area and in flagellar membranes of sperm	Strongylocentrotus purpuratus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Strongylocentrotus purpuratus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	degraded sAC, Western blot analysis	Strongylocentrotus purpuratus
190000	-	Western blot analysis	Strongylocentrotus purpuratus

Organism

Organism	UniProt	Comment	Textmining
Strongylocentrotus purpuratus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially purified	Strongylocentrotus purpuratus

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	flagellum	Strongylocentrotus purpuratus	-
sperm	both tmAC and sAC present, presence of more than one tmAC isoform in sea urchin sperm	Strongylocentrotus purpuratus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Strongylocentrotus purpuratus	3',5'-cAMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
particulate adenylyl cyclase	-	Strongylocentrotus purpuratus
sAC	-	Strongylocentrotus purpuratus
soluble adenylyl cyclase	-	Strongylocentrotus purpuratus
tmAC	-	Strongylocentrotus purpuratus
transmembrane adenylyl cyclase	-	Strongylocentrotus purpuratus

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Release 2023.1 (January 2023)