Literature summary for 4.6.1.1 extracted from

Smith, N.; Kim, S.K.; Reddy, P.T.; Gallagher, D.T.
Crystallization of the class IV adenylyl cyclase from Yersinia pestis (2006), Acta Crystallogr. Sect. F, 62, 200-204.

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into pET15b vector, recombinant plasmid isolated from Escherichia coli NovaBlue cells and introduced into Escherichia coli BL21(DE3) cells for protein expression	Yersinia pestis

Crystallization (Commentary)

Crystallization (Comment)	Organism
by vapour diffusion-hanging drop method, amino-terminal His-tagged construct, from which the tag was removed by thrombin, crystallized in a triclinic form diffracting to 1.9 A resolution, with one dimer per asymmetric unit and unit-cell parameters a = 33.5, b = 35.5, c = 71.8 A, alpha = 88.7, beta = 82.5, gamma = 65.5°. Non-His tagged native construct crystallized in space group P212121 with unit-cell parameters a = 56.8, b = 118.6, c = 144.5 A, to 2.9 A resolution and probably having two dimers per asymmetric unit	Yersinia pestis

Crystallization (Comment)

Organism

by vapour diffusion-hanging drop method, amino-terminal His-tagged construct, from which the tag was removed by thrombin, crystallized in a triclinic form diffracting to 1.9 A resolution, with one dimer per asymmetric unit and unit-cell parameters a = 33.5, b = 35.5, c = 71.8 A, alpha = 88.7, beta = 82.5, gamma = 65.5°. Non-His tagged native construct crystallized in space group P212121 with unit-cell parameters a = 56.8, b = 118.6, c = 144.5 A, to 2.9 A resolution and probably having two dimers per asymmetric unit

Yersinia pestis

Protein Variants

Protein Variants	Comment	Organism
D143A	does not crystallize	Yersinia pestis
E10A	gives crystals that are unsuitable owing to poor crystal growth or poor diffraction	Yersinia pestis
E136A	gives crystals that are unsuitable owing to poor crystal growth or poor diffraction	Yersinia pestis
E84A	gives crystals that are unsuitable owing to poor crystal growth or poor diffraction	Yersinia pestis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20500	-	non-His tagged native construct	Yersinia pestis
40000	-	gel filtration	Yersinia pestis

Organism

Organism	UniProt	Comment	Textmining
Yersinia pestis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
non-His tagged native construct, on NiNTA resin and by gel filtration	Yersinia pestis

Subunits

Subunits	Comment	Organism
dimer	gel filtration	Yersinia pestis

Synonyms

Synonyms	Comment	Organism
class IV adenylyl cyclase	-	Yersinia pestis

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	crystal forms of the native and His tagged enzyme appear to require pH below 5	Yersinia pestis