Cloned (Comment) | Organism |
---|---|
expression of cytosolic catalytic domain in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D365C | 0.06% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type | Mycobacterium tuberculosis |
K296E | 0.5% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type | Mycobacterium tuberculosis |
K296E/D365C | 0.025% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type | Mycobacterium tuberculosis |
K296E/F363R/D365C | 0.5% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | 6 transmembrane helices and a single cytosolic catalytic domain, which dimerizes to form a 12-transmembrane homodimeric enzyme | Mycobacterium tuberculosis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | Mycobacterium tuberculosis | - |
3',5'-cyclic AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant cytosolic catalytic domain | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Mycobacterium tuberculosis | 3',5'-cyclic AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Mycobacterium tuberculosis |
Ry1625c | - |
Mycobacterium tuberculosis |