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Literature summary for 4.5.1.B1 extracted from

  • Macwan, A.S.; Kukshal, V.; Srivastava, N.; Javed, S.; Kumar, A.; Ramachandran, R.
    Crystal structure of the hexachlorocyclohexane dehydrochlorinase (LinA-type2): mutational analysis, thermostability and enantioselectivity (2012), PLoS ONE, 7, e50373.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.5.1.B1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,3,4,5,6-pentachlorocyclohexene uncultured bacterium
-
 1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
-
 ?
gamma-hexachlorocyclohexane uncultured bacterium
-
 pentachlorocyclohexene + HCl
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium B5ANU3 LinA-type 2
-
uncultured bacterium B5ANV6 LinA-type 1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3,4,5,6-pentachlorocyclohexene
-
 uncultured bacterium 1,3,4,6-tetrachloro-1,4-cyclohexadiene + HCl
-
 ?
gamma-hexachlorocyclohexane
-
 uncultured bacterium pentachlorocyclohexene + HCl
-
 ?
gamma-hexachlorocyclohexane activity differs with isomers alpha, gamma, and delta, LinA-type1 exhibits enantioselectivity for the (-)-enantiomer of alpha-hexachlorocyclohexane uncultured bacterium pentachlorocyclohexene + HCl
-
 ?
gamma-hexachlorocyclohexane activity differs with isomers alpha, gamma, and delta, LinA-type2 exhibits enantioselectivity for the (+)-enantiomer of alpha-hexachlorocyclohexane uncultured bacterium pentachlorocyclohexene + HCl
-
 ?

Synonyms

Synonyms Comment Organism
LinA-type1
-
 uncultured bacterium
LinA-type2
-
 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 residues Q20 and G23 are determinants of thermostability for LinA-type2 uncultured bacterium

General Information

General Information Comment Organism
evolution changed residues K20Q, L96C and A131G, vicinal to the active site, are probably responsible for the altered enantioselectivity of LinA-type2 compared to LinA-type1, structural comparison of LinA-type2 and -type1 proteins suggests that thermostability of LinA-type2 might partly arise due to presence of higher number of ionic interactions, along with 4% increase in the intersubunit buried surface area uncultured bacterium
metabolism the enzyme mediates the first step of aerobic degradation of a chlorinated insecticide gamma-hexachlorocyclohexane uncultured bacterium
additional information active site structure, structure comparison and docking modeling, overview uncultured bacterium