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Literature summary for 4.5.1.3 extracted from
- Identification and cloning of a gene encoding dichloromethane dehalogenase from a methylotrophic bacterium, Bacillus circulans WZ-12 CCTCC M 207006 (2009), Bioprocess Biosyst. Eng., 32, 845-852.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Niallia circulans |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) from a pET28b(+) expression system | Niallia circulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
Dichloromethane | - |
Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
SDS-PAGE | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dichloromethane + H2O | Niallia circulans | - |
formaldehyde + 2 Cl- | - |
? | |
| dichloromethane + H2O | Niallia circulans WZ-12 CCTCC M 207006 | - |
formaldehyde + 2 Cl- | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | B7TXX4 | - |
- |
| Niallia circulans WZ-12 CCTCC M 207006 | B7TXX4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation, Q-Sepharose Fast Flow column, hydroxylapatite column, Mono-Q GL5/5 column, 4°C | Niallia circulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108.1 | - |
- |
Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dichloromethane + H2O | - |
Niallia circulans | formaldehyde + 2 Cl- | - |
? | |
| dichloromethane + H2O | - |
Niallia circulans WZ-12 CCTCC M 207006 | formaldehyde + 2 Cl- | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000 | Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DehalA | - |
Niallia circulans |
| dichloromethane dehalogenase | - |
Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 7.0 | Niallia circulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | pH 7.0 | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | more than 50% of the activity is observed between 25°C and 40°C | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
30°C | Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | 30°C | Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | the enzyme retains more than 50% activity between pH 6.0 and 7.5 but the activity drops off markedly at a pH value above 7.5 or below 6.0 | Niallia circulans |
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