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Literature summary for 4.5.1.3 extracted from

  • Leisinger, T.; Kohler-Staub, D.
    Dichloromethane dehalogenase from Hyphomicobium DM2 (1990), Methods Enzymol., 188, 355-361.
No PubMed abstract available
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione required Hyphomicrobium sp.
glutathione Km: 0.32 mM Hyphomicrobium sp.
glutathione sulfhydryl compounds such as 2-mercaptoethanol, DTT or cysteine cannot substitute for reduced glutathione Hyphomicrobium sp.

Inhibitors

Inhibitors Comment Organism Structure
1,2-Dichloroethane
-
 Hyphomicrobium sp.
1-Chloropropane
-
 Hyphomicrobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 Diiodomethane 30°C, pH 8.2 Hyphomicrobium sp.
0.013
-
 Dibromomethane 30°C, pH 8.2 Hyphomicrobium sp.
0.015
-
 CH2BrCl 30°C, pH 8.2 Hyphomicrobium sp.
0.03
-
 Dichloromethane 30°C, pH 8.2 Hyphomicrobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 alpha6, 6 * 33000, SDS-PAGE Hyphomicrobium sp.
195000
-
 gel filtration Hyphomicrobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Hyphomicrobium sp.
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM2 Hyphomicrobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.041
-
 hydroxylapatite eluate Hyphomicrobium sp.

Storage Stability

Storage Stability Organism
-20°C, protein concentration 4 mg/ml, 100 mM potassium phosphate, pH 7.5, 2.5 mM DTT, 0.1 mM EDTA, 25% v/v glycerol, stable for 6 months Hyphomicrobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH2BrCl + H2O
-
 Hyphomicrobium sp. formaldehyde + Br- + Cl-
-
 ?
dibromomethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Br-
-
 ?
dichloromethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Cl-
-
 ?
diiodomethane + H2O
-
 Hyphomicrobium sp. formaldehyde + I-
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha6, 6 * 33000, SDS-PAGE Hyphomicrobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hyphomicrobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 6 days, 50% loss of activity Hyphomicrobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Hyphomicrobium sp.