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Literature summary for 4.5.1.2 extracted from
- 3-Chloro-D-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1-1 (1984), Prog. Clin. Biol. Res., 144A, 365-375.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Pseudomonas putida |  |
| CuSO3 | - |
Pseudomonas putida | |
| cycloserine | - |
Pseudomonas putida | |
| D-penicillamine | - |
Pseudomonas putida | |
| HgCl2 | - |
Pseudomonas putida | |
| iodoacetate | - |
Pseudomonas putida | |
| KCN | - |
Pseudomonas putida | |
| phenylhydrazine | - |
Pseudomonas putida | |
| thiol reagents | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
3-chloro-D-alanine | - |
Pseudomonas putida | |
| 0.18 | - |
D-cysteine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Pseudomonas putida |
| 76000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chloro-D-alanine + H2O | Pseudomonas putida | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
CR1-1, AKU 867 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CR1-1 | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 310 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chloro-D-alanine + 2-mercaptoethanol | - |
Pseudomonas putida | S-(2-hydroxyethyl)-D-cysteine | - |
? | |
| 3-chloro-D-alanine + allyl mercaptane | - |
Pseudomonas putida | S-allyl-D-cysteine | - |
? | |
| 3-chloro-D-alanine + alpha-thiolglycerol | - |
Pseudomonas putida | S-(2,3-dihydroxypropyl)-D-cysteine | - |
? | |
| 3-chloro-D-alanine + ethyl-mercaptane | - |
Pseudomonas putida | S-ethyl-D-cysteine | - |
? | |
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida | pyruvate + HCl + NH3 | - |
ir | |
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3-chloro-D-alanine + methyl-mercaptane | - |
Pseudomonas putida | S-methyl-D-cysteine | - |
? | |
| 3-chloro-D-alanine + n-butyl-mercaptane | - |
Pseudomonas putida | S-n-butyl-D-cysteine | - |
? | |
| 3-chloro-D-alanine + n-propyl-mercaptane | - |
Pseudomonas putida | S-n-propyl-D-cysteine | - |
? | |
| 3-chloro-D-alanine + NaHS | - |
Pseudomonas putida | D-cysteine + NaCl | - |
ir | |
| 3-chloro-D-alanine + phenyl-mercaptane | - |
Pseudomonas putida | S-phenyl-D-cysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
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