BRENDA - Enzyme Database
show all sequences of 4.5.1.2

Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of Pseudomonas putida

Yamada, H.; Nagasawa, T.; Ohkishi, H.; Kawakami, B.; Tani, Y.; Biochem. Biophys. Res. Commun. 100, 1104-1110 (1981)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8.1
-
3-chloro-D-alanine
-
Pseudomonas putida
46
-
H2S
-
Pseudomonas putida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
soluble
-
Pseudomonas putida
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-chloro-D-alanine + H2O
Pseudomonas putida
-
?
-
-
-
3-chloro-D-alanine + H2O
Pseudomonas putida CR1-1
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
CR1-1
-
Pseudomonas putida CR1-1
-
CR1-1
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida
pyruvate + HCl + NH3
-
34745
Pseudomonas putida
ir
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida CR1-1
pyruvate + HCl + NH3
-
34745
Pseudomonas putida CR1-1
ir
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida
?
-
-
-
-
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida CR1-1
?
-
-
-
-
3-chloro-D-alanine + H2S
-
34745
Pseudomonas putida
D-cysteine + HCl
-
34745
Pseudomonas putida
ir
3-chloro-D-alanine + H2S
-
34745
Pseudomonas putida CR1-1
D-cysteine + HCl
-
34745
Pseudomonas putida CR1-1
ir
additional information
specific for 3-chloro-D-alanine
34745
Pseudomonas putida
?
-
34745
Pseudomonas putida
-
additional information
specific for 3-chloro-D-alanine
34745
Pseudomonas putida CR1-1
?
-
34745
Pseudomonas putida CR1-1
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
cysteine synthesis
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8.1
-
3-chloro-D-alanine
-
Pseudomonas putida
46
-
H2S
-
Pseudomonas putida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
soluble
-
Pseudomonas putida
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-chloro-D-alanine + H2O
Pseudomonas putida
-
?
-
-
-
3-chloro-D-alanine + H2O
Pseudomonas putida CR1-1
-
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida
pyruvate + HCl + NH3
-
34745
Pseudomonas putida
ir
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida CR1-1
pyruvate + HCl + NH3
-
34745
Pseudomonas putida CR1-1
ir
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida
?
-
-
-
-
3-chloro-D-alanine + H2O
-
34745
Pseudomonas putida CR1-1
?
-
-
-
-
3-chloro-D-alanine + H2S
-
34745
Pseudomonas putida
D-cysteine + HCl
-
34745
Pseudomonas putida
ir
3-chloro-D-alanine + H2S
-
34745
Pseudomonas putida CR1-1
D-cysteine + HCl
-
34745
Pseudomonas putida CR1-1
ir
additional information
specific for 3-chloro-D-alanine
34745
Pseudomonas putida
?
-
34745
Pseudomonas putida
-
additional information
specific for 3-chloro-D-alanine
34745
Pseudomonas putida CR1-1
?
-
34745
Pseudomonas putida CR1-1
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
cysteine synthesis
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Pseudomonas putida
Other publictions for EC 4.5.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730698
Bharath
Structural and mutational stud ...
Salmonella enterica subsp. enterica serovar Typhimurium
PLoS ONE
7
e36267
2012
-
-
1
1
7
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
7
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
34752
Nagasawa
Physiological comparison of D- ...
Pseudomonas putida
Arch. Microbiol.
149
413-416
1988
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34753
Nagasawa
3-Chloro-D-alanine chloride-ly ...
Pseudomonas putida
Prog. Clin. Biol. Res.
144A
365-375
1984
-
-
-
1
-
-
9
2
-
-
2
1
-
2
-
-
1
-
-
-
1
-
11
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
9
-
2
-
-
2
1
-
-
-
1
-
-
1
-
11
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
34746
Nagasawa
Synthesis of S-(carboxymethyl) ...
Pseudomonas putida, Pseudomonas putida CR1-1
Appl. Biochem. Biotechnol.
8
481-489
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34747
Soda
-
Synthesis of D-cysteine by a n ...
Pseudomonas putida, Pseudomonas putida CR1-1
Trends Biochem. Sci.
8
152-153
1983
-
-
-
1
-
-
-
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
2
2
-
-
-
-
-
-
1
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
34748
Nagasawa
Synthesis of D-cysteine-relate ...
Pseudomonas putida, Pseudomonas putida CR1-1
Biochem. Biophys. Res. Commun.
111
809-816
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34749
Nagasawa
-
Synthesis of C-cysteine from a ...
Pseudomonas putida, Pseudomonas putida CR1-1
Agric. Biol. Chem.
47
861-868
1983
-
-
-
-
-
-
1
-
-
-
-
-
-
7
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34750
Nagasawa
-
Enzymatic synthesis of D-cyste ...
Pseudomonas putida, Pseudomonas putida CR1-1
Agric. Biol. Chem.
46
3003-3010
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
34751
Nagasawa
3-Chloro-D-alanine chloride-ly ...
Pseudomonas putida
J. Biol. Chem.
257
13749-13756
1982
-
-
-
1
-
-
13
1
-
-
2
-
-
3
-
-
1
-
-
-
1
-
-
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
1
-
-
-
13
-
1
-
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
34745
Yamada
Synthesis of D-cysteine from 3 ...
Pseudomonas putida, Pseudomonas putida CR1-1
Biochem. Biophys. Res. Commun.
100
1104-1110
1981
-
-
-
-
-
-
-
2
2
-
-
2
-
7
-
-
-
-
-
-
1
-
8
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
2
-
-
2
-
-
-
-
-
-
1
-
8
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-