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Literature summary for 4.4.1.9 extracted from
- A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning (2014), eLife, 3, e02365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Tu-CAS , DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli | Tetranychus urticae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.312 | - |
L-cysteine | pH 8.5, 37°C, recombinant His-tagged enzyme | Tetranychus urticae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Tetranychus urticae | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + hydrogen cyanide | Tetranychus urticae | - |
hydrogen sulfide + L-3-cyanoalanine | - |
? | |
| additional information | Tetranychus urticae | the enzyme is also active with O-acetyl-L-serine, EC 2.5.1.47, but the L-3-cyanoalanine forming activity is highly preferred | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetranychus urticae | T1KF23 | cv. Prelude, gene Tu-CAS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Tetranychus urticae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.84 | - |
pH 8.5, 37°C, purified recombinant His-tagged enzyme | Tetranychus urticae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + hydrogen cyanide | - |
Tetranychus urticae | hydrogen sulfide + L-3-cyanoalanine | - |
? | |
| additional information | the enzyme is also active with O-acetyl-L-serine, EC 2.5.1.47, but the L-3-cyanoalanine forming activity is highly preferred | Tetranychus urticae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAS | - |
Tetranychus urticae |
| Tu-CAS | - |
Tetranychus urticae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Tetranychus urticae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2135 | - |
L-cysteine | pH 8.5, 37°C, recombinant His-tagged enzyme | Tetranychus urticae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Tetranychus urticae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Tetranychus urticae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the gene encoding the enzyme was transferred to the genome of the spider mite Tetranychus urticae perhaps a few hundred million years ago, it is horizontally acquired from bacteria. An equivalent gene is also found in moths and butterflies, which explains why these insects can thrive on plants that produce hydrogen cyanide. Lateral gene transfer from bacteria to animals, overview | Tetranychus urticae |
| physiological function | the enzyme is responsible for detoxification of cyanide to beta-cyanoalanine in the spider mite | Tetranychus urticae |
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