Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.9 extracted from

  • Ikegami, F.; Takayama, K.; Kurihara, T.; Horiuchi, S.; Tajima, C.; Shirai, R.; Murakoshi, I.
    Purification and properties of beta-cyano-L-alanine synthase from Vicia angustifolia (1989), Phytochemistry, 28, 2285-2291.
No PubMed abstract available
Search for more literature for 4.4.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 KCN
-
 Lupinus sp.
0.5
-
 cyanide
-
 Vicia sativa subsp. nigra
0.66
-
 2-mercaptoethanol
-
 Lupinus sp.
0.87
-
 cysteine
-
 Lupinus sp.
3.6
-
 L-cysteine
-
 Vicia sativa subsp. nigra
10
-
 2-mercaptoethanol
-
 Lupinus sp.
55
-
 KCN
-
 Lupinus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000 53000 gel filtration Lupinus sp.
54000
-
 gel filtration Vicia sativa subsp. nigra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + HCN Vicia sativa subsp. nigra
-
 ?
-
 ?
L-cysteine + HCN Lupinus sp. detoxification of cyanide in plants and first step of L-asparagine biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lupinus sp.
-
-
-
Vicia sativa subsp. nigra
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vicia sativa subsp. nigra

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Vicia sativa subsp. nigra
-
seedling
-
 Lupinus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
 Vicia sativa subsp. nigra

Storage Stability

Storage Stability Organism
stable at -20°C Lupinus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-L-alanine + HCN
-
 Lupinus sp. ?
-
 ir
L-cysteine + 2-mercaptoethanol
-
 Lupinus sp. ?
-
 ?
L-cysteine + HCN
-
 Lupinus sp. H2S + L-3-cyanoalanine
-
 ir
L-cysteine + HCN
-
 Vicia sativa subsp. nigra ?
-
 ?
L-cysteine + HCN detoxification of cyanide in plants and first step of L-asparagine biosynthesis Lupinus sp. ?
-
 ?
L-cysteine + KCN
-
 Lupinus sp. L-3-cyanoalanin + K2S
-
 ?
L-cysteine + methanethiol
-
 Vicia sativa subsp. nigra S-methyl-L-cysteine + ?
-
 ?
additional information
-
 Lupinus sp. ?
-
 ?
additional information
-
 Vicia sativa subsp. nigra ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Lupinus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9.5
-
 Lupinus sp.
9.4 9.5
-
 Vicia sativa subsp. nigra

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate firmly bound Lupinus sp.