Cloned (Comment) | Organism |
---|---|
overexpressing mutant YEpGLO1, shows increased methylglyoxal resistance | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTAglo1 | glyoxalase I null mutant | Saccharomyces cerevisiae |
YEpGLO1 | overexpressing mutant, shows increased methylglyoxal resistance | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | methylglyoxal toxicity is evaluated by viability assays using BY4741 and the null mutant DELTAglo1, lacking the glyoxalase I activity. The reference BY4741 strain shows no methylglyxoal toxicity up to 10 mM. In contrast, strains with deficiencies in the glyoxalase system, i.e. DELTAglo1 have decreased viability, progessively more severe with increasing methylglyoxal concentration | Saccharomyces cerevisiae | |
phosphate | 750 mM, strain BY4741, about 2fold decrease of glyoxalase I. In the YEpGLO1 strain, glyoxalase is higher than in BY4741, consistant with the overexpression of the glo1 gene. Enzyme inactivation is observed, cells subjected to 750 mM phosphate still show an increase of about 1.7fold relatively to BY4741 glyoxalase I activity | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains BY4741, null mutant DELTAglo1, and overexpressed strain YEpGLO1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + glutathione | permeabilized cell suspensions, 30°C, pH 7.1 | Saccharomyces cerevisiae | S-((R)-lactoyl)glutathione | formation is monitored by measuring the increase at 240 nm | ? |
Synonyms | Comment | Organism |
---|---|---|
glyoxalase I | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Saccharomyces cerevisiae |