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Literature summary for 4.4.1.5 extracted from
- Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes (2007), Biochim. Biophys. Acta, 1774, 756-763.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | Pseudomonas aeruginosa possesses GlxI enzymes from two metal activation classes. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene encodes a Zn2+-dependent homolog | Pseudomonas aeruginosa PAO1 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gloA2 and gloA3 ligated into vector pET22b, overexpression in Escherichia coli BL21(gammaDE3) | Pseudomonas aeruginosa PAO1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | decreases GloA3 activity | Pseudomonas aeruginosa PAO1 |  |
| dipicolinic acid | greatest loss of GloA3 activity | Pseudomonas aeruginosa PAO1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | reactivates GloA3 by 87% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
| Co2+ | increases GloA2 activity, hyper-reactivates GloA3 by 115% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
| Mg2+ | reactivates GloA3 by 26% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
| Mn2+ | hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
| Ni2+ | increases GloA2 activity, hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
| Zn2+ | does not increase GloA2 activity, GloA3 contains Zn2+, reactivates GloA3 by 76% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa PAO1 | Q9HU72 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GloA2 and GloA3, by gel filtration | Pseudomonas aeruginosa PAO1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + methylglyoxal | - |
Pseudomonas aeruginosa PAO1 | S-D-lactoylglutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | GloA2 and GloA3, gel filtration | Pseudomonas aeruginosa PAO1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GloA1 | - |
Pseudomonas aeruginosa PAO1 |
| GloA2 | - |
Pseudomonas aeruginosa PAO1 |
| GloA3 | - |
Pseudomonas aeruginosa PAO1 |
| GLXI | - |
Pseudomonas aeruginosa PAO1 |
| glyoxalase I | - |
Pseudomonas aeruginosa PAO1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Pseudomonas aeruginosa PAO1 |
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Release 2023.1 (January 2023)
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