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Literature summary for 4.4.1.5 extracted from
- Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombe (2004), Arch. Microbiol., 181, 371-377.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Schizosaccharomyces pombe |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
Phenylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe |  |
| 0.8 | - |
glutathione | pH 7.0, 25°C | Schizosaccharomyces pombe | |
| 1.6 | - |
methylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20mM, 32.8% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Co2+ | 1mM, 78.6% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Fe2+ | 1mM, 8.4% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Mg2+ | 20mM, 13.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Mn2+ | 1mM, 186% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Ni2+ | 1mM, 67.9% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
| Zn2+ | 0.5mM, 84.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
PR 109 | - |
| Schizosaccharomyces pombe PR 109 | - |
PR 109 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax methylglyoxal purified enzyme 2 mmol/min/mg protein, Vmax phenylglyoxal 0.197 mmol/min/mg protein, pH 7.0, 25°C | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + methylglyoxal | - |
Schizosaccharomyces pombe | (R)-S-lactoylglutathione | - |
? | |
| glutathione + methylglyoxal | - |
Schizosaccharomyces pombe PR 109 | (R)-S-lactoylglutathione | - |
? | |
| glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe | S-mandeloylglutathione | - |
? | |
| glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe PR 109 | S-mandeloylglutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glo1 | - |
Schizosaccharomyces pombe |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
100% activity after 4 h incubation at 60°C, 50% inactivation after 150 min incubation at 64°C or after 60 min incubation at 65°C | Schizosaccharomyces pombe |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | stable at 4°C for at least 24h | Schizosaccharomyces pombe |
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