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Literature summary for 4.4.1.5 extracted from

  • Sommer, A.; Fischer, P.; Krause, K.; Boettcher, K.; Brophy, P.M.; Walter, R.D.; Liebau, E.
    A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus (2001), Biochem. J., 353, 445-452.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Onchocerca volvulus

Inhibitors

Inhibitors Comment Organism Structure
dichlorophen 0.048 mM, 50% inhibition Onchocerca volvulus
formononetin 7-O-glucoside 0.074 mM, 50% inhibition Onchocerca volvulus
oxibendazol 0.11 mM, 50% inhibition Onchocerca volvulus
purpurogallin 0.015 mM, 50% inhibition Onchocerca volvulus
S-hexylglutathione 0.11 mM, 50% inhibition Onchocerca volvulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 Phenylglyoxal
-
 Onchocerca volvulus
0.34
-
 methylglyoxal
-
 Onchocerca volvulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 2 * 24000, SDS-PAGE Onchocerca volvulus
45000
-
 gel filtration Onchocerca volvulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione-methylglyoxal hemithioacetal Onchocerca volvulus glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?

Organism

Organism UniProt Comment Textmining
Onchocerca volvulus
-
 causative agent of onchocerciasis, GloI expression is induced by oxidative stress
-

Purification (Commentary)

Purification (Comment) Organism
recombinant glyoxalase I Onchocerca volvulus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
 towards phenylglyoxal Onchocerca volvulus
284
-
 towards methylglyoxal Onchocerca volvulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + methylglyoxal
-
 Onchocerca volvulus S-D-lactoylglutathione
-
 ?
glutathione + phenylglyoxal
-
 Onchocerca volvulus S-mandeloylglutathione
-
 ?
glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Onchocerca volvulus (R)-S-lactoylglutathione
-
 ?
glutathione-phenylglyoxal hemithioacetal glutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from phenylglyoxal and glutathione Onchocerca volvulus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Onchocerca volvulus

Synonyms

Synonyms Comment Organism
GloI
-
 Onchocerca volvulus
glyoxalase I
-
 Onchocerca volvulus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 Phenylglyoxal
-
 Onchocerca volvulus
11.4
-
 methylglyoxal
-
 Onchocerca volvulus
81.7
-
 Phenylglyoxal
-
 Onchocerca volvulus