KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0272 | - |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14919 | - |
2 * 14919, electrospray mass spectrometry | Escherichia coli |
14990 | - |
2 * 14990, SDS-PAGE | Escherichia coli |
30000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | ? | - |
? | |
additional information | Escherichia coli MG1655/pGL10 | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AC81 | MG1655/pGL10 | - |
Escherichia coli MG1655/pGL10 | P0AC81 | MG1655/pGL10 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + methylglyoxal | the substrat is the hemithioacetal of methylglyoxal and glutathione | Escherichia coli | S-lactoylglutathione | - |
? | |
glutathione + methylglyoxal | the substrat is the hemithioacetal of methylglyoxal and glutathione | Escherichia coli MG1655/pGL10 | S-lactoylglutathione | - |
? | |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli | (R)-S-lactoylglutathione | - |
? | |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli MG1655/pGL10 | (R)-S-lactoylglutathione | - |
? | |
additional information | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | Escherichia coli | ? | - |
? | |
additional information | the enzyme is a part of the glyoxalase system that is composed of EC 4.4.1.5 and EC 3.1.2.6. The glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids | Escherichia coli MG1655/pGL10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14990, SDS-PAGE | Escherichia coli |
dimer | 2 * 14919, electrospray mass spectrometry | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
338 | - |
glutathione-methylglyoxal hemithioacetal | - |
Escherichia coli |