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Literature summary for 4.4.1.5 extracted from

  • Baskaran, S.; Balasubramanian, K.A.
    Purification and active site modification studies on glyoxalase I from monkey intestinal mucosa (1987), Biochim. Biophys. Acta, 913, 377-385.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
Macaca radiata
2,4,6-Trinitrobenzenesulfonic acid
-
Macaca radiata
2-Hydroxy-5-nitrobenzyl bromide
-
Macaca radiata
diethyl dicarbonate
-
Macaca radiata
I- 5 mM, complete inhibition Macaca radiata
N-Acetylimidazole reversed by hydroxylamine Macaca radiata
N-bromosuccinimide
-
Macaca radiata
NO3- 10 mM, 20% decrease of activity Macaca radiata
pyridoxal 5'-phosphate
-
Macaca radiata
S-hexylglutathione competitive Macaca radiata
Tetranitromethane
-
Macaca radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM restores 25% of the activity of the apoenzyme Macaca radiata
Co2+ 1 mM, increases activity more than 100%. 1 mM restores 80% of the activity of the apoenzyme Macaca radiata
Mg2+ 1 mM restores 60% of the activity of the apoenzyme Macaca radiata
Mn2+ 1 mM restores 35% of the activity of the apoenzyme Macaca radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
2 * 24000, SDS-PAGE Macaca radiata
48000
-
gel filtration Macaca radiata

Organism

Organism UniProt Comment Textmining
Macaca radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Macaca radiata

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
Macaca radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
925
-
-
Macaca radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + methylglyoxal
-
Macaca radiata S-lactoylglutathione
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Macaca radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Macaca radiata