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Literature summary for 4.4.1.4 extracted from

  • Chhabria, S.; Desai, K.
    Purification and characterisation of alliinase produced by Cupriavidus necator and its application for generation of cytotoxic agent allicin (2018), Saudi J. Biol. Sci., 25, 1429-1438 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.83
-
 alliin pH 7.0, 30°C Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel fitration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
209
-
 pH 7.0, 30°C Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alliin
-
 Cupriavidus necator allylsulfenic acid + pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Cupriavidus necator

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 80% of maximum activity Cupriavidus necator
40
-
 80% of maximum activity Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 80% of maximum activity Cupriavidus necator
8
-
 80% of maximum activity Cupriavidus necator

Expression

Organism Comment Expression
Cupriavidus necator maximum alliinase production is observed at growth at 37°C and at pH 7 additional information