Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
C115A | mutation leads to a decrease of the catalytic efficiency in the gamma-elimination reaction of the physiological substrate by more than an order of magnitude, reation of EC 4.4.1.11 | Citrobacter freundii |
C115H | mutation leads to loss of activity in the gamma-elimination reaction of the physiological substrate, reaction of EC 4.4.1.11. The catalytic efficiency in the beta-elimination reaction of S-substituted L-cysteine sulfoxides is increased by about an order of magnitude compared to the wild type | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
alliin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
0.457 | - |
ethiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
0.57 | - |
methiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
0.63 | - |
methiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
1.75 | - |
alliin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
5.48 | - |
ethiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | Q84AR1 | cf. EC 4.4.1.11 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alliin + H2O | - |
Citrobacter freundii | allicin + pyruvate + NH3 | - |
? | |
ethiin + H2O | - |
Citrobacter freundii | ethanesulfenic acid + pyruvate + NH3 | - |
? | |
methiin + H2O | - |
Citrobacter freundii | methanesulfenic acid + pyruvate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
alliin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
1.25 | - |
ethiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
1.63 | - |
methiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
5.14 | - |
methiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
5.59 | - |
ethiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
11.31 | - |
alliin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
alliin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
0.23 | - |
ethiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
2.6 | - |
methiin | mutant C115A, pH 8.0, 30°C | Citrobacter freundii | |
9.02 | - |
methiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
12 | - |
ethiin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii | |
25.7 | - |
alliin | mutant C115H, pH 8.0, 30°C | Citrobacter freundii |