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Literature summary for 4.4.1.4 extracted from

  • Morozova, E.; Kulikova, V.; Rodionov, A.; Revtovich, S.; Anufrieva, N.; Demidkina, T.
    Engineered Citrobacter freundii methionine gamma-lyase effectively produces antimicrobial thiosulfinates (2016), Biochimie, 128-129, 92-98 .
    View publication on PubMed
Search for more literature for 4.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
C115A mutation leads to a decrease of the catalytic efficiency in the gamma-elimination reaction of the physiological substrate by more than an order of magnitude, reation of EC 4.4.1.11 Citrobacter freundii
C115H mutation leads to loss of activity in the gamma-elimination reaction of the physiological substrate, reaction of EC 4.4.1.11. The catalytic efficiency in the beta-elimination reaction of S-substituted L-cysteine sulfoxides is increased by about an order of magnitude compared to the wild type Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.457
-
 ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.57
-
 methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.63
-
 methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.75
-
 alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
5.48
-
 ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q84AR1 cf. EC 4.4.1.11
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
ethiin + H2O
-
 Citrobacter freundii ethanesulfenic acid + pyruvate + NH3
-
 ?
methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.25
-
 ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.63
-
 methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
5.14
-
 methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
5.59
-
 ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
11.31
-
 alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
 alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.23
-
 ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
2.6
-
 methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
9.02
-
 methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
12
-
 ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
25.7
-
 alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii