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Literature summary for 4.4.1.4 extracted from

  • Rabinkov, A.; Zhu, X.Z.; Grafi, G.; Galili, G.; Mirelman, D.
    Alliin lyase (Alliinase) from garlic (Allium sativum). Biochemical characterization and cDNA cloning (1994), Appl. Biochem. Biotechnol., 48, 149-171.
    View publication on PubMed
Search for more literature for 4.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Xenopus leavis oocytes Allium sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 S-allyl-L-Cys sulfoxide
-
 Allium sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51400
-
 2 * 51400, SDS-PAGE Allium sativum
90000
-
-
 Allium sativum

Organism

Organism UniProt Comment Textmining
Allium sativum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Allium sativum
side-chain modification contains 6% carbohydrate Allium sativum

Purification (Commentary)

Purification (Comment) Organism
-
 Allium sativum

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Allium sativum
-
clove
-
 Allium sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
260
-
-
 Allium sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-allyl-L-cysteine sulfoxide + H2O
-
 Allium sativum 2-propene-thioic acid + pyruvate + NH4+ + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51400, SDS-PAGE Allium sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Allium sativum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium sativum