Literature summary for 4.4.1.37 extracted from

  • Fellner, M.; Desguin, B.; Hausinger, R.; Hu, J.
    Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE (2017), Proc. Natl. Acad. Sci. USA, 114, 9074-9079 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Lactococcus lactis and Escherichia coli Arctic-Express (DE3) cells Lactiplantibacillus plantarum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 105 mM ammonium sulfate, 50 mM bis-Tris pH 6.5, 37.5 % (v/v) pentaerythritol ethoxylate Lactiplantibacillus plantarum

Engineering

Protein Variants Comment Organism
D30A inactive Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in form of Mg-ATP Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP Lactiplantibacillus plantarum
-
[LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP Lactiplantibacillus plantarum
-
[LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP Lactiplantibacillus plantarum ATCC BAA-793
-
[LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP Lactiplantibacillus plantarum ATCC BAA-793
-
[LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum F9UST4
-
-
Lactiplantibacillus plantarum ATCC BAA-793 F9UST4
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-tactin column chromatography and Superdex 200 gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP
-
Lactiplantibacillus plantarum [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP
-
Lactiplantibacillus plantarum ATCC BAA-793 [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP
-
Lactiplantibacillus plantarum [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP
-
Lactiplantibacillus plantarum ATCC BAA-793 [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
LarE
-
Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Lactiplantibacillus plantarum