Cloned (Comment) | Organism |
---|---|
expressed in Lactococcus lactis and Escherichia coli Arctic-Express (DE3) cells | Lactiplantibacillus plantarum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 105 mM ammonium sulfate, 50 mM bis-Tris pH 6.5, 37.5 % (v/v) pentaerythritol ethoxylate | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
D30A | inactive | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required in form of Mg-ATP | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP | Lactiplantibacillus plantarum | - |
[LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP | Lactiplantibacillus plantarum ATCC BAA-793 | - |
[LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP | Lactiplantibacillus plantarum | - |
[LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP | Lactiplantibacillus plantarum ATCC BAA-793 | - |
[LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | F9UST4 | - |
- |
Lactiplantibacillus plantarum ATCC BAA-793 | F9UST4 | - |
- |
Purification (Comment) | Organism |
---|---|
Strep-tactin column chromatography and Superdex 200 gel filtration | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP | - |
Lactiplantibacillus plantarum | [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP | - |
Lactiplantibacillus plantarum ATCC BAA-793 | [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP | - |
Lactiplantibacillus plantarum | [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate | - |
? | |
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP | - |
Lactiplantibacillus plantarum ATCC BAA-793 | [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LarE | - |
Lactiplantibacillus plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Lactiplantibacillus plantarum |