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Literature summary for 4.4.1.37 extracted from

  • Desguin, B.; Soumillion, P.; Hols, P.; Hausinger, R.
    Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion (2016), Proc. Natl. Acad. Sci. USA, 113, 5598-5603 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Lactococcus lactis Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in form of Mg-ATP Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP Lactiplantibacillus plantarum overall reaction [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
 ?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP Lactiplantibacillus plantarum overall reaction [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin column chromatography, and Superdex 200 gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[LarE]-L-cysteine + pyridin-1-ium-3,5-dicarboxylate mononucleotide + ATP overall reaction Lactiplantibacillus plantarum [LarE]-dehydroalanine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + AMP + diphosphate
-
 ?
[LarE]-L-cysteine + pyridin-1-ium-3-carboxylate-5-thiocarboxylate mononucleotide + ATP overall reaction Lactiplantibacillus plantarum [LarE]-dehydroalanine + pyridin-1-ium-3,5-bisthiocarboxylate mononucleotide + AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 31550, calculated from amino acid sequence Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
LarE
-
 Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Lactiplantibacillus plantarum