Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.36 extracted from

  • Song, H.; Hu, W.; Naowarojna, N.; Her, A.S.; Wang, S.; Desai, R.; Qin, L.; Chen, X.; Liu, P.
    Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis the involvement of a sulfenic acid intermediate (2015), Sci. Rep., 5, 11870 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.36

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.121
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.96
-
 (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R5M7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + reduced acceptor thioether variant of S-(hercyn-2-yl)-L-cysteine S-oxide Mycolicibacterium smegmatis (2R)-3-(2-sulfido-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + pyruvate + ammonia + acceptor
-
 ?
2-(hydroxysulfanyl)hercynine + reduced acceptor
-
 Mycolicibacterium smegmatis ergothioneine + acceptor + H2O
-
 ?
additional information S-(hercyn-2-yl)-L-cysteine S-oxide is the native EgtE substrate and a sulfenic acid intermediate is involved in the ergothioneine C-S lyase reaction Mycolicibacterium smegmatis ?
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 Mycolicibacterium smegmatis 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor
-
 Mycolicibacterium smegmatis ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Synonyms

Synonyms Comment Organism
egtE
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.9
-
 (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis
25.3
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.8 mol per mol of monomer. At pH 7.5, absorption near 420 nm, i.e. presence of ketoenamine tautomer Mycolicibacterium smegmatis