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Literature summary for 4.4.1.36 extracted from

  • Pluskal, T.; Ueno, M.; Yanagida, M.
    Genetic and metabolomic dissection of the ergothioneine and selenoneine biosynthetic pathway in the fission yeast, S. pombe, and construction of an overproduction system (2014), PLoS ONE, 9, e97774 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.36

Cloned(Commentary)

Cloned (Comment) Organism
gene egt2+, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information construction of an egt2+ overexpression system by replacing its native promoter with the nmt1+ promoter, which is inducible in the absence of thiamine. Generation of a egt2+ deletion mutant, DELTAegt2, by replacing the target loci in the wild-type 972 strain with the kanamycin resistance marker (kanMX). Mutant DELTASPBC660.12c, designated DELTAegt2, shows a substantial decrease in ergothioneine, accompanied by accumulation of its immediate precursor, hercynylcysteine sulfoxide. Ergothioneine-deficient strains exhibit no phenotypic defects during vegetative growth or quiescence. Construction of multiple deletion mutants of SPBC660.12c (egt2+) with the other candidate EgtE homologues, but even in successfully constructed double and triple mutants, a significant amount of EGT still remains, because hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'phosphate. Mutant DELTAegt1 strain shows no growth defects during cultivation in either rich (YE) or minimal (EMM2) culture media, deletion of gene egt1+ causes no significant perturbation to the intracellular metabolome of quiescent cells Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead ?
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additional information Schizosaccharomyces pombe 972 the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead ?
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 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Schizosaccharomyces pombe overall reaction ergothioneine + pyruvate + ammonia + acceptor
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S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor Schizosaccharomyces pombe 972 overall reaction ergothioneine + pyruvate + ammonia + acceptor
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 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O94431
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Schizosaccharomyces pombe 972 O94431
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead Schizosaccharomyces pombe ?
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 ?
additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead Schizosaccharomyces pombe 972 ?
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 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Schizosaccharomyces pombe ergothioneine + pyruvate + ammonia + acceptor
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 ?
S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor overall reaction Schizosaccharomyces pombe 972 ergothioneine + pyruvate + ammonia + acceptor
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 ?

Subunits

Subunits Comment Organism
More Egt-2 domain structure, overview Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Egt-2
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 Schizosaccharomyces pombe
Egt2
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 Schizosaccharomyces pombe
egt2+
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 Schizosaccharomyces pombe
egtE
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 Schizosaccharomyces pombe
ergothioneine biosynthesis protein 2
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 Schizosaccharomyces pombe
hercynylcysteine sulfoxide lyase UniProt Schizosaccharomyces pombe
SPBC660.12c locus name Schizosaccharomyces pombe

General Information

General Information Comment Organism
metabolism SPBC660.12c locus egt2+ represents the gene primarily responsible for the second step of ergothioneine biosynthesis in Schizosaccharomyces pombe Schizosaccharomyces pombe
physiological function a gene deletion mutant shows a substantial decrease in ergothioneine, accompanied by accumulation of its immediate precursor, hercynylcysteine sulfoxide. Ergothioneine-deficient strains exhibit no phenotypic defects during vegetative growth or quiescence. The ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium Schizosaccharomyces pombe