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Literature summary for 4.4.1.35 extracted from

  • Jones, P.R.; Manabe, T.; Awazuhara, M.; Saito, K.
    A new member of plant CS-lyases. A cystine lyase from Arabidopsis thaliana (2003), J. Biol. Chem., 278, 10291-10296.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.32
-
L-cystine pH 7.6, 22°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
-
Brassica oleracea
47200
-
-
Brassica oleracea

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SUR6
-
-
Brassica oleracea Q84UD0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica oleracea
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystine + H2O
-
Arabidopsis thaliana L-thiocysteine + pyruvate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE, two bands of two closely related isoforms. 2 * 47200, calculated Brassica oleracea

Synonyms

Synonyms Comment Organism
BOCL3
-
Brassica oleracea
CORI3
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Brassica oleracea calculated
-
8.3