Application | Comment | Organism |
---|---|---|
synthesis | production of of a fluorescent holo-C-phycocyanin subunit alpha in Escherichia coli by construction of an expression vector containing five essential genes in charge of biosynthesis of cyanobacterial C-phycocyanin holo-alpha subunit. The vector harbors two cassettes: one cassette carries genes hox1 and pcyA required for conversion of heme to phycocyanobilin, and the other cassette carries cpcA encoding C-phycocyanin subunit alpha along with lyase subunits cpcE and cpcF both of which are necessary and sufficient for the correct addition of phycobiliprotein to CpcA. The maximum peak of absorbance spectrum is at 623 nm, and the maximum peak of fluorescence emission and excitation are at 648 and 633 nm, respectively, which are similar to those of native C-phycocyanin | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | P73638 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[C-phycocyanin alpha-subunit] + phycocyanobilin | - |
Synechocystis sp. | [C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cpcE | - |
Synechocystis sp. |