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Literature summary for 4.4.1.30 extracted from

  • Zhang, J.; Sun, Y.; Zhao, K.; Zhou, M.
    Identification of amino acid residues essential to the activity of lyase CpcT1 from Nostoc sp. PCC7120 (2012), Gene, 511, 88-95.
    View publication on PubMed
Search for more literature for 4.4.1.30

Cloned(Commentary)

Cloned (Comment) Organism
-
 Nostoc sp.

Protein Variants

Protein Variants Comment Organism
C116S in vitro assay,11% of wild-type activity Nostoc sp.
C137S in vitro assay, 11% of wild-type activity Nostoc sp.
H33F in vitro assay, 14% of wild-type activity Nostoc sp.
additional information chemical modification of arginine, histidine, tryptophan, lysine and amino acid carboxyl groups of isoform CpcT1 decreases activity to 30-84% of wild-type Nostoc sp.
R66A in vitro assay, 15% of wild-type activity Nostoc sp.
R97A in vitro assay, 37% of wild-type activity. Contrary to wild-type,a great deal of alpha helix is involved in circular dichroism Nostoc sp.
W13S in vitro assay, 22% of wild-type activity. Contrary to wild-type,a great deal of alpha helix is involved in circular dichroism Nostoc sp.
W175S in vitro assay, 14% of wild-type activity Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp. Q8YLF9
-
-
Nostoc sp. PCC 7120 Q8YLF9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apo-[phycocyanin beta-subunit] + phycocyanobilin
-
 Nostoc sp. [phycocyanin beta-subunit]-Cys155-phycocyanobilin
-
 ?
apo-[phycocyanin beta-subunit] + phycocyanobilin
-
 Nostoc sp. PCC 7120 [phycocyanin beta-subunit]-Cys155-phycocyanobilin
-
 ?
additional information isoform CpcT1 cannot bind phycobilin Nostoc sp. ?
-
 ?
additional information isoform CpcT1 cannot bind phycobilin Nostoc sp. PCC 7120 ?
-
 ?

Synonyms

Synonyms Comment Organism
CpcT1
-
 Nostoc sp.