Literature summary for 4.4.1.3 extracted from

Li, C.Y.; Zhang, D.; Chen, X.L.; Wang, P.; Shi, W.L.; Li, P.Y.; Zhang, X.Y.; Qin, Q.L.; Todd, J.D.; Zhang, Y.Z.
Mechanistic insights into dimethylsulfoniopropionate lyase DddY, a new member of the cupin superfamily (2017), J. Mol. Biol., 429, 3850-3862 .

Search for more literature for 4.4.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acinetobacter bereziniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.5 A resolution. DddY contains a cap domain and a catalytic domain with a Zn2+ bound at its active site. Residues His265, Glu269, His338 and the acetate molecule participate in coordinating Zn2+. The DddY catalytic domain adopts a typical beta-barrel fold and contains two conserved cupin motifs. In the catalytic mechanism, residue Tyr271 acts as a general base to attack DMSP	Acinetobacter bereziniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5	-	beta-dimethylsulfoniopropanoate	pH 8.0, 60°C	Acinetobacter bereziniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	Zn2+ is bound at the active site	Acinetobacter bereziniae

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter bereziniae	N8X9V6	-	-
Acinetobacter bereziniae NIPH 3	N8X9V6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-dimethylsulfoniopropanoate	-	Acinetobacter bereziniae	dimethylsulfide + acrylate	-	?
beta-dimethylsulfoniopropanoate	-	Acinetobacter bereziniae NIPH 3	dimethylsulfide + acrylate	-	?

Synonyms

Synonyms	Comment	Organism
Dddy	-	Acinetobacter bereziniae
F963_02809	-	Acinetobacter bereziniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Acinetobacter bereziniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8300	-	beta-dimethylsulfoniopropanoate	pH 8.0, 60°C	Acinetobacter bereziniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Acinetobacter bereziniae

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Release 2023.1 (January 2023)