Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Alcaligenes faecalis |
expression in Escherichia coli | Halodesulfovibrio aestuarii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine | selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity | Alcaligenes faecalis | |
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine | selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity | Halodesulfovibrio aestuarii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Halodesulfovibrio aestuarii | |
2.56 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Alcaligenes faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Alcaligenes faecalis | |
Ca2+ | - |
Halodesulfovibrio aestuarii | |
Mn2+ | - |
Alcaligenes faecalis | |
Mn2+ | - |
Halodesulfovibrio aestuarii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | E7DDH2 | - |
- |
Halodesulfovibrio aestuarii | A0A8G2CC70 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
902 | - |
pH 8, 30°C | Alcaligenes faecalis |
1391 | - |
pH 8, 30°C | Halodesulfovibrio aestuarii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-dimethylsulfoniopropanoate | - |
Alcaligenes faecalis | dimethylsulfide + acrylate | - |
? | |
beta-dimethylsulfoniopropanoate | - |
Halodesulfovibrio aestuarii | dimethylsulfide + acrylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dddy | - |
Alcaligenes faecalis |
Dddy | - |
Halodesulfovibrio aestuarii |
SAMN05660830_03106 | - |
Halodesulfovibrio aestuarii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
900 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Alcaligenes faecalis | |
1130 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Halodesulfovibrio aestuarii |
General Information | Comment | Organism |
---|---|---|
physiological function | comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis | Alcaligenes faecalis |
physiological function | comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis | Halodesulfovibrio aestuarii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
350 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Alcaligenes faecalis | |
1320 | - |
beta-dimethylsulfoniopropanoate | pH 8.0, 30°C | Halodesulfovibrio aestuarii |