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Literature summary for 4.4.1.3 extracted from

  • Lei, L.; Cherukuri, K.P.; Alcolombri, U.; Meltzer, D.; Tawfik, D.S.
    The dimethylsulfoniopropionate (DMSP) lyase and lyase-like cupin family consists of bona fide DMSP lyases as well as other enzymes with unknown function (2018), Biochemistry, 57, 3364-3377 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alcaligenes faecalis
expression in Escherichia coli Halodesulfovibrio aestuarii

Inhibitors

Inhibitors Comment Organism Structure
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity Alcaligenes faecalis
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity Halodesulfovibrio aestuarii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii
2.56
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Alcaligenes faecalis
Ca2+
-
Halodesulfovibrio aestuarii
Mn2+
-
Alcaligenes faecalis
Mn2+
-
Halodesulfovibrio aestuarii

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis E7DDH2
-
-
Halodesulfovibrio aestuarii A0A8G2CC70
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
902
-
pH 8, 30°C Alcaligenes faecalis
1391
-
pH 8, 30°C Halodesulfovibrio aestuarii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-dimethylsulfoniopropanoate
-
Alcaligenes faecalis dimethylsulfide + acrylate
-
?
beta-dimethylsulfoniopropanoate
-
Halodesulfovibrio aestuarii dimethylsulfide + acrylate
-
?

Synonyms

Synonyms Comment Organism
Dddy
-
Alcaligenes faecalis
Dddy
-
Halodesulfovibrio aestuarii
SAMN05660830_03106
-
Halodesulfovibrio aestuarii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
900
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis
1130
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii

General Information

General Information Comment Organism
physiological function comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis Alcaligenes faecalis
physiological function comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis Halodesulfovibrio aestuarii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
350
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis
1320
-
beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii