Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Roseovarius nubinhibens |
Protein Variants | Comment | Organism |
---|---|---|
D295A | complete loss of activity | Roseovarius nubinhibens |
D297A | complete loss of activity | Roseovarius nubinhibens |
D307A | complete loss of activity | Roseovarius nubinhibens |
E406A | complete loss of activity | Roseovarius nubinhibens |
E421A | complete loss of activity | Roseovarius nubinhibens |
H371A | complete loss of activity | Roseovarius nubinhibens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM | Roseovarius nubinhibens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
beta-dimethylsulfoniopropanoate | pH 6.0, 30°C | Roseovarius nubinhibens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels | Roseovarius nubinhibens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49972 | - |
2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
50000 | - |
2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
95300 | - |
sedimentation equilibrium ultracentrifugation | Roseovarius nubinhibens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Roseovarius nubinhibens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Roseovarius nubinhibens |
Storage Stability | Organism |
---|---|
4°C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks | Roseovarius nubinhibens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-dimethylsulfoniopropanoate | - |
Roseovarius nubinhibens | dimethylsulfide + acrylate | - |
? | |
additional information | enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor | Roseovarius nubinhibens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49972, calculated, 2 * 50000, SDS-PAGE | Roseovarius nubinhibens |
Synonyms | Comment | Organism |
---|---|---|
dddP | - |
Roseovarius nubinhibens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Roseovarius nubinhibens |
55 | 60 | - |
Roseovarius nubinhibens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
rapid loss of activity above | Roseovarius nubinhibens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Roseovarius nubinhibens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | more than 70% of maximum activity | Roseovarius nubinhibens |