BRENDA - Enzyme Database
show all sequences of 4.4.1.3

The dddP gene of Roseovarius nubinhibens encodes a novel lyase that cleaves dimethylsulfoniopropionate into acrylate plus dimethyl sulfide

Kirkwood, M.; Le Brun, N.E.; Todd, J.D.; Johnston, A.W.; Microbiology 156, 1900-1906 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Roseovarius nubinhibens
Engineering
Amino acid exchange
Commentary
Organism
D295A
complete loss of activity
Roseovarius nubinhibens
D297A
complete loss of activity
Roseovarius nubinhibens
D307A
complete loss of activity
Roseovarius nubinhibens
E406A
complete loss of activity
Roseovarius nubinhibens
E421A
complete loss of activity
Roseovarius nubinhibens
H371A
complete loss of activity
Roseovarius nubinhibens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM
Roseovarius nubinhibens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14
-
beta-dimethylsulfoniopropanoate
pH 6.0, 30°C
Roseovarius nubinhibens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels
Roseovarius nubinhibens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49972
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
50000
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
95300
-
sedimentation equilibrium ultracentrifugation
Roseovarius nubinhibens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Roseovarius nubinhibens
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Roseovarius nubinhibens
Storage Stability
Storage Stability
Organism
4°C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks
Roseovarius nubinhibens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-dimethylsulfoniopropanoate
-
716137
Roseovarius nubinhibens
dimethylsulfide + acrylate
-
-
-
?
additional information
enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor
716137
Roseovarius nubinhibens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Roseovarius nubinhibens
55
60
-
Roseovarius nubinhibens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
rapid loss of activity above
Roseovarius nubinhibens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Roseovarius nubinhibens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
7
more than 70% of maximum activity
Roseovarius nubinhibens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Roseovarius nubinhibens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D295A
complete loss of activity
Roseovarius nubinhibens
D297A
complete loss of activity
Roseovarius nubinhibens
D307A
complete loss of activity
Roseovarius nubinhibens
E406A
complete loss of activity
Roseovarius nubinhibens
E421A
complete loss of activity
Roseovarius nubinhibens
H371A
complete loss of activity
Roseovarius nubinhibens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM
Roseovarius nubinhibens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14
-
beta-dimethylsulfoniopropanoate
pH 6.0, 30°C
Roseovarius nubinhibens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels
Roseovarius nubinhibens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49972
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
50000
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
95300
-
sedimentation equilibrium ultracentrifugation
Roseovarius nubinhibens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Roseovarius nubinhibens
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks
Roseovarius nubinhibens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-dimethylsulfoniopropanoate
-
716137
Roseovarius nubinhibens
dimethylsulfide + acrylate
-
-
-
?
additional information
enzyme is member of the M24B family, which acts on a non-peptide substrate and has no metal cofactor
716137
Roseovarius nubinhibens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 49972, calculated, 2 * 50000, SDS-PAGE
Roseovarius nubinhibens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Roseovarius nubinhibens
55
60
-
Roseovarius nubinhibens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
rapid loss of activity above
Roseovarius nubinhibens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Roseovarius nubinhibens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
7
more than 70% of maximum activity
Roseovarius nubinhibens
Other publictions for EC 4.4.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747144
Lei
The dimethylsulfoniopropionat ...
Alcaligenes faecalis, Halodesulfovibrio aestuarii
Biochemistry
57
3364-3377
2018
-
-
2
-
-
-
2
2
-
4
-
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3
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2
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2
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2
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2
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2
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2
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4
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2
-
2
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2
-
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-
-
-
2
2
-
2
2
748278
Wang
-
How is DMSP decomposed when c ...
Ruegeria lacuscaerulensis, Ruegeria lacuscaerulensis DSM 11314
J. Catal.
360
1-8
2018
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2
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1
1
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-
748399
Li
Mechanistic insights into dim ...
Acinetobacter bereziniae, Acinetobacter bereziniae NIPH 3
J. Mol. Biol.
429
3850-3862
2017
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-
1
1
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1
-
1
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4
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2
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1
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1
1
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1
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1
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1
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1
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2
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
748814
Burkhardt
Chemical differentiation of t ...
Phaeobacter inhibens, Phaeobacter inhibens DSM 17395, Ruegeria pomeroyi, Ruegeria pomeroyi DSM 15171
Org. Biomol. Chem.
15
4432-4439
2017
-
-
-
-
-
-
-
18
-
-
-
-
-
8
-
-
-
-
-
-
-
-
33
-
-
-
-
18
-
-
-
-
-
-
-
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-
-
-
-
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-
-
18
-
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33
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
747110
Brummett
New mechanistic insight from ...
Ruegeria lacuscaerulensis, Ruegeria lacuscaerulensis DSM 11314
Biochemistry
55
6162-6174
2016
-
-
-
1
-
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1
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2
-
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1
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1
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1
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1
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748685
Wang
Structural and molecular basi ...
Ruegeria lacuscaerulensis, Ruegeria lacuscaerulensis DSM 11314
Mol. Microbiol.
98
289-301
2015
-
-
1
1
3
-
3
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
1
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-
1
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1
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1
3
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3
-
1
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-
4
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
749057
Brummett
Biochemical, kinetic, and spe ...
Ruegeria pomeroyi, Ruegeria pomeroyi DSM 15171
PLoS ONE
10
e0127288
2015
-
-
1
-
-
-
-
2
-
5
2
-
-
9
-
-
1
-
-
-
2
-
2
1
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
5
2
-
-
-
-
1
-
-
2
-
2
1
-
-
-
2
1
-
-
-
-
-
-
-
2
2
730958
Conway
Co-cultures of Pseudomonas aer ...
Roseobacter denitrificans, Roseobacter denitrificans Och114
ScientificWorldJournal
2012
120108
2012
-
-
1
-
-
-
-
-
-
-
-
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-
2
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1
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-
-
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-
-
-
-
1
-
-
1
-
-
716093
Franklin
-
Dimethylsulphoniopropionate (D ...
Emiliania huxleyi, Gephyrocapsa oceanica
Mar. Ecol. Prog. Ser.
410
13-23
2010
-
-
-
-
2
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-
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2
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1
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2
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1
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-
716137
Kirkwood
The dddP gene of Roseovarius n ...
Roseovarius nubinhibens
Microbiology
156
1900-1906
2010
-
-
1
-
6
-
1
1
-
1
3
-
-
4
-
-
1
-
-
-
-
1
2
1
2
-
1
-
1
1
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1
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6
-
-
1
-
1
-
1
3
-
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-
1
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-
1
2
1
2
-
1
-
1
1
-
-
-
-
-
-
-
-
692099
Curson
Molecular genetic analysis of ...
Fulvimarina pelagi HTCC2506, Pseudooceanicola batsensis HTCC2597, Rhodobacter sphaeroides, Stappia sp., Stappia sp. KB902, Sulfitobacter sp. EE-36, Yoonia vestfoldensis
Environ. Microbiol.
10
1099
2008
-
-
6
-
-
-
-
-
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-
-
7
-
8
-
-
-
-
-
-
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-
14
-
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6
-
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7
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-
14
-
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-
697645
Curson
Molecular genetic analysis of ...
Fulvimarina pelagi, Labrenzia aggregata IAM 12614, Pseudooceanicola batsensis, Rhodobacter sphaeroides 2.4.1, Rhodobacter sphaeroides ATCC 17025, Rhodobacter sphaeroides ATCC 17029, Sulfitobacter sp. EE-36, Yoonia vestfoldensis
Environ. Microbiol.
10
757-767
2008
-
-
1
-
1
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-
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-
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17
-
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8
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1
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1
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-
8
-
-
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-
-
-
-
-
-
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-
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-
653010
Wolfe
-
Dimethylsulfoniopropionate cle ...
Alexandrium ssp., Alexandrium ssp. Halim, Emiliania huxleyi
J. Phycol.
38
948-960
2002
4
-
-
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3
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4
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649363
Ansede
Nuclear magnetic resonance ana ...
unidentified proteobacterium
Appl. Environ. Microbiol.
67
3134-3139
2001
-
-
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2
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1
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653049
Steinke
-
Determinations of dimethylsulf ...
Emiliania huxleyi
J. Sea Res.
43
233-244
2000
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1
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1
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1
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-
653106
Niki
-
DMSP lyase activity in five ma ...
Heterocapsa triquetra, no activity in Gephyrocapsa oceanica, no activity in Heterosigma akashiwo, no activity in Isochrysis galbana, Scrippsiella trochoidea
Mar. Biol.
136
759-764
2000
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5
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2
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2
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-
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649351
Ansede
Metabolism of acrylate to beta ...
Alcaligenes faecalis
Appl. Environ. Microbiol.
65
5075-5081
1999
-
-
-
-
-
-
-
-
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-
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4
-
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1
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1
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1
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34603
Bacic
In vivo characterization of di ...
Fusarium lateritium
Appl. Environ. Microbiol.
64
106-111
1998
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-
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2
1
1
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1
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4
-
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2
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1
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1
1
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2
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1
1
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1
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2
-
1
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1
1
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34602
Ansede
-
Comparison of selenium and sul ...
Alcaligenes sp., Alcaligenes sp. M3A, Oceanimonas doudoroffii
FEMS Microbiol. Ecol.
23
315-324
1997
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-
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2
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3
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6
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9
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2
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3
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9
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34604
Yoch
Evidence for intracellular and ...
Alcaligenes sp., Alcaligenes sp. M3A, Oceanimonas doudoroffii
Appl. Environ. Microbiol.
63
3182-3188
1997
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3
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2
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10
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3
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34607
De Souza
-
Dimethylsulfoniopropionate lya ...
Ulva curvata
Planta
199
433-438
1996
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
1
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34605
De Souza
Purification and characterizat ...
Alcaligenes sp., Alcaligenes sp. M3A
Appl. Environ. Microbiol.
61
21-26
1995
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-
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1
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1
2
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7
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1
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1
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4
1
1
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1
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1
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1
2
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1
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1
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4
1
1
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1
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34606
De Souza
Comparative physiology of dime ...
Alcaligenes sp., Alcaligenes sp. M3A, Oceanimonas doudoroffii
Appl. Environ. Microbiol.
61
3986-3991
1995
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6
2
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4
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9
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7
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6
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2
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4
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7
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34601
Cantoni
Enzymatic cleavage of dimethyl ...
Vertebrata lanosa
J. Biol. Chem.
222
171-177
1956
1
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