Protein Variants | Comment | Organism |
---|---|---|
C51T | insoluble mutant enzyme | Anabaena sp. |
C63T | kcat/Km for (2R,3E)-phycocyanobilin is 1.2fold lower than wild-type value | Anabaena sp. |
G119C/W140V | insoluble mutant enzyme | Anabaena sp. |
H21T/H22V | kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value | Anabaena sp. |
H22V | kcat/Km for (2R,3E)-phycocyanobilin is 2.5fold lower than wild-type value | Anabaena sp. |
H80L | kcat/Km for (2R,3E)-phycocyanobilin is 1.5fold lower than wild-type value | Anabaena sp. |
R147Q | kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value | Anabaena sp. |
R169L | kcat/Km for (2R,3E)-phycocyanobilin is 1.7fold lower than wild-type value, loss of fidelity in chromophore attachment | Anabaena sp. |
R18V | kcat/Km for (2R,3E)-phycocyanobilin is 7.5fold lower than wild-type value | Anabaena sp. |
W14I | insoluble mutant enzyme | Anabaena sp. |
W69M | kcat/Km for (2R,3E)-phycocyanobilin is 1.1fold higher than wild-type value | Anabaena sp. |
W75S | kcat/Km for (2R,3E)-phycocyanobilin is 3fold lower than wild-type value | Anabaena sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | non-competitive | Anabaena sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00114 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, wild-type enzyme | Anabaena sp. | |
0.00134 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H21T/H22V | Anabaena sp. | |
0.00138 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H22V | Anabaena sp. | |
0.00139 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R169L | Anabaena sp. | |
0.00139 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W69M | Anabaena sp. | |
0.00144 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W75S | Anabaena sp. | |
0.00155 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme C63T | Anabaena sp. | |
0.0017 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H80L | Anabaena sp. | |
0.00229 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R147Q | Anabaena sp. | |
0.00254 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R18V | Anabaena sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. | - |
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | Anabaena sp. PCC 7120 | - |
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | Q8YZ70 | - |
- |
Anabaena sp. PCC 7120 | Q8YZ70 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | - |
Anabaena sp. | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? | |
apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin | - |
Anabaena sp. PCC 7120 | [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpcS1 | - |
Anabaena sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Anabaena sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000073 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R18V | Anabaena sp. | |
0.0000095 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H21T/H22V | Anabaena sp. | |
0.0000106 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W75S | Anabaena sp. | |
0.0000119 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H22V | Anabaena sp. | |
0.0000169 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R147Q | Anabaena sp. | |
0.000018 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R169L | Anabaena sp. | |
0.0000246 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H80L | Anabaena sp. | |
0.000025 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, wild-type enzyme | Anabaena sp. | |
0.000028 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme C63T | Anabaena sp. | |
0.0000343 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W69M | Anabaena sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Anabaena sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R18V | Anabaena sp. | |
0.0071 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H21T/H22V | Anabaena sp. | |
0.0074 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R147Q | Anabaena sp. | |
0.0074 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W75S | Anabaena sp. | |
0.0086 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H22V | Anabaena sp. | |
0.0129 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme R169L | Anabaena sp. | |
0.0147 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme H80L | Anabaena sp. | |
0.018 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme C63T | Anabaena sp. | |
0.0219 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, wild-type enzyme | Anabaena sp. | |
0.0247 | - |
(2R,3E)-phycocyanobilin | pH 7.5, 37°C, mutant enzyme W69M | Anabaena sp. |