Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactobacillus delbrueckii subsp. bulgaricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
S-aminoethyl-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
0.4 | - |
L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
0.48 | - |
L-djenkolic acid | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
0.5 | - |
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
2.1 | - |
L-cystine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
4 | - |
L-cystathionine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration | Lactobacillus delbrueckii subsp. bulgaricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii subsp. bulgaricus | A0A0U5KG73 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | A0A0U5KG73 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Lactobacillus delbrueckii subsp. bulgaricus |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus | NH3 + pyruvate + homocysteine | - |
? | |
L-cystathionine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | NH3 + pyruvate + homocysteine | - |
? | |
L-cysteine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | sulfide + NH3 + pyruvate | - |
? | |
L-cystine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus | ? | - |
? | |
L-cystine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | ? | - |
? | |
L-djenkolic acid | - |
Lactobacillus delbrueckii subsp. bulgaricus | ? | - |
? | |
additional information | the enzyme shows a clear preference for aminoethyl-L-cysteine together with L-cystine and the non-protein amino acid L-djenkolate. Enzyme additionally acts as cysteine-S-conjugate beta-lyase in vitro, reaction of EC 4.4.1.13, and is able to cleave a cysteinylated substrate precursor into the corresponding thiol. Enzyme has no alpha,gamma-elimination activity towards L-methionine to produce methanethiol | Lactobacillus delbrueckii subsp. bulgaricus | ? | - |
? | |
additional information | the enzyme shows a clear preference for aminoethyl-L-cysteine together with L-cystine and the non-protein amino acid L-djenkolate. Enzyme additionally acts as cysteine-S-conjugate beta-lyase in vitro, reaction of EC 4.4.1.13, and is able to cleave a cysteinylated substrate precursor into the corresponding thiol. Enzyme has no alpha,gamma-elimination activity towards L-methionine to produce methanethiol | Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | ? | - |
? | |
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus | NH3 + pyruvate + 4-methyl-4-sulfanylpentan-2-one | - |
? | |
S-aminoethyl-L-cysteine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus | NH3 + pyruvate + cysteamine | - |
? | |
S-aminoethyl-L-cysteine + H2O | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 | NH3 + pyruvate + cysteamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
Synonyms | Comment | Organism |
---|---|---|
CFL1_01513 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
thermal inactivation midpoint | Lactobacillus delbrueckii subsp. bulgaricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
4 | - |
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
11 | - |
L-cystathionine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
17.8 | - |
S-aminoethyl-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
19 | - |
L-djenkolic acid | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
29 | - |
L-cystine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Lactobacillus delbrueckii subsp. bulgaricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-cystathionine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
7 | - |
L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
8 | - |
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
14 | - |
L-cystine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
40 | - |
L-djenkolic acid | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus | |
66 | - |
S-aminoethyl-L-cysteine | pH 8.3, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |