Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.28 extracted from

  • Allegrini, A.; Astegno, A.; La Verde, V.; Dominici, P.
    Characterization of C-S lyase from Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 and its potential role in food flavour applications (2017), J. Biochem., 161, 349-360 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
S-aminoethyl-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
0.4
-
L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
0.48
-
L-djenkolic acid pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
0.5
-
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
2.1
-
L-cystine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
4
-
L-cystathionine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
gel filtration Lactobacillus delbrueckii subsp. bulgaricus

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. bulgaricus A0A0U5KG73
-
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 A0A0U5KG73
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Lactobacillus delbrueckii subsp. bulgaricus

Storage Stability

Storage Stability Organism
-20°C, stable for several months Lactobacillus delbrueckii subsp. bulgaricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus NH3 + pyruvate + homocysteine
-
?
L-cystathionine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 NH3 + pyruvate + homocysteine
-
?
L-cysteine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 sulfide + NH3 + pyruvate
-
?
L-cystine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus ?
-
?
L-cystine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 ?
-
?
L-djenkolic acid
-
Lactobacillus delbrueckii subsp. bulgaricus ?
-
?
additional information the enzyme shows a clear preference for aminoethyl-L-cysteine together with L-cystine and the non-protein amino acid L-djenkolate. Enzyme additionally acts as cysteine-S-conjugate beta-lyase in vitro, reaction of EC 4.4.1.13, and is able to cleave a cysteinylated substrate precursor into the corresponding thiol. Enzyme has no alpha,gamma-elimination activity towards L-methionine to produce methanethiol Lactobacillus delbrueckii subsp. bulgaricus ?
-
?
additional information the enzyme shows a clear preference for aminoethyl-L-cysteine together with L-cystine and the non-protein amino acid L-djenkolate. Enzyme additionally acts as cysteine-S-conjugate beta-lyase in vitro, reaction of EC 4.4.1.13, and is able to cleave a cysteinylated substrate precursor into the corresponding thiol. Enzyme has no alpha,gamma-elimination activity towards L-methionine to produce methanethiol Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 ?
-
?
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus NH3 + pyruvate + 4-methyl-4-sulfanylpentan-2-one
-
?
S-aminoethyl-L-cysteine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus NH3 + pyruvate + cysteamine
-
?
S-aminoethyl-L-cysteine + H2O
-
Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 NH3 + pyruvate + cysteamine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Lactobacillus delbrueckii subsp. bulgaricus

Synonyms

Synonyms Comment Organism
CFL1_01513
-
Lactobacillus delbrueckii subsp. bulgaricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Lactobacillus delbrueckii subsp. bulgaricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
thermal inactivation midpoint Lactobacillus delbrueckii subsp. bulgaricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
4
-
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
11
-
L-cystathionine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
17.8
-
S-aminoethyl-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
19
-
L-djenkolic acid pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
29
-
L-cystine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Lactobacillus delbrueckii subsp. bulgaricus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Lactobacillus delbrueckii subsp. bulgaricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
L-cystathionine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
7
-
L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
8
-
S-(2-methyl-4-oxopentan-2-yl)-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
14
-
L-cystine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
40
-
L-djenkolic acid pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus
66
-
S-aminoethyl-L-cysteine pH 8.3, 30°C Lactobacillus delbrueckii subsp. bulgaricus