Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cyanoborohydride | complete inactivation | Treponema denticola | |
glycine | competitive inhibitor | Treponema denticola | |
L-alanine | competitive inhibitor | Treponema denticola | |
L-tryptophan | competitive inhibitor | Treponema denticola | |
pyruvate | - |
Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
S-ethyl-L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
0.36 | - |
beta-chloro-L-alanine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
0.4 | - |
L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
1.26 | - |
S-methyl-L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
2.3 | - |
L-cysteine | pH and temperature not specified in the publication, in absence of Brij 35 | Treponema denticola | |
62 | - |
L-cysteine methyl ester | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | Treponema denticola | the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola | sulfide + NH3 + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-alanine + H2O | - |
Treponema denticola | sulfide + ? | - |
? | |
L-cysteine + H2O | the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola | Treponema denticola | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | strong preference for L-cysteine over D-cysteine, H2S production is 483-fold slower with D-cysteine than with L-cysteine. It is far more active towards L-cysteine than towards the other standard amino acids that undergo pyridoxal phosphate-dependent beta-elimination reactions (serine, threonine, tryptophan and tyrosine). The enzyme tolerated small modifications to the carboxylate of L-cysteine (i.e., the methyl and ethyl esters of L-cysteine are good substrates), but the smallest possible peptide with an N-terminal cysteine, L-cysteinylglycine, is a very poor substrate | Treponema denticola | sulfide + NH3 + pyruvate | - |
? | |
L-cysteine methyl ester + H2O | - |
Treponema denticola | sulfide + ? | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Treponema denticola | sulfide + ? | - |
? | |
S-methyl-L-cysteine + H2O | - |
Treponema denticola | sulfide + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cystalysin | - |
Treponema denticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
5.95 | - |
S-methyl-L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
9.5 | - |
S-ethyl-L-cysteine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
10.9 | - |
L-cysteine | pH and temperature not specified in the publication, in absence of Brij 35 | Treponema denticola | |
14.5 | - |
beta-chloro-L-alanine | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola | |
32 | - |
L-cysteine methyl ester | pH and temperature not specified in the publication, addition of Brij 35 to the assay buffer | Treponema denticola |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Treponema denticola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
L-tryptophan | pH and temperature not specified in the publication, in absence of Brij 35 | Treponema denticola |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is responsible for in vivo cysteine catabolism by Treponema denticola. Unregulated enzyme, with the resulting H2S production being essential to the hemolytic activity | Treponema denticola |