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Literature summary for 4.4.1.28 extracted from
- Cloning and characterization of the L-cysteine desulfhydrase gene of Fusobacterium nucleatum (2002), FEMS Microbiol. Lett., 215, 75-80.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a His6-tagged fusion protein | Fusobacterium nucleatum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.122 | - |
L-cysteine | pH 7.5, 37°C, recombinant enzyme purified from Escherichia coli | Fusobacterium nucleatum |  |
| 0.155 | - |
L-cysteine | pH 7.5, 37°C, enzyme purified from Fusobacterium nucleatum | Fusobacterium nucleatum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, enzyme purified from Fusobacterium nucleatum | Fusobacterium nucleatum |
| 33400 | - |
x * 33400, calculated from sequence | Fusobacterium nucleatum |
| 37000 | - |
x * 37000, SDS-PAGE, recombinant His6-tagged fusion protein | Fusobacterium nucleatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusobacterium nucleatum | Q8L0X3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme purified form Fusobacterium nucleatum cell extract and from recombinant Escherichia coli | Fusobacterium nucleatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + H2O | no activity with DLK-homocysteine, D-cysteine, L-cystine and cystathionine | Fusobacterium nucleatum | sulfide + NH3 + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, enzyme purified from Fusobacterium nucleatum | Fusobacterium nucleatum |
| ? | x * 33400, calculated from sequence | Fusobacterium nucleatum |
| ? | x * 37000, SDS-PAGE, recombinant His6-tagged fusion protein | Fusobacterium nucleatum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDL | - |
Fusobacterium nucleatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Fusobacterium nucleatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Fusobacterium nucleatum |
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