Literature summary for 4.4.1.26 extracted from

Yang, X.; Matsui, T.; Kodama, T.; Mori, T.; Zhou, X.; Taura, F.; Noguchi, H.; Abe, I.; Morita, H.
Structural basis for olivetolic acid formation by a polyketide cyclase from Cannabis sativa (2016), FEBS J., 283, 1088-1106 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Cannabis sativa

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of free enzyme and in complex with olivetolic acid, to 1.32 and 1.70 A resolutions. The active-site cavity contains the pentyl-binding hydrophobic pocket and the polyketide binding site. Residues Tyr72 and His78 function as acid/base catalysts at the catalytic center. The enzyme may lack thioesterase and aromatase activities	Cannabis sativa

Protein Variants

Protein Variants	Comment	Organism
F24L	49% of wild-type activity	Cannabis sativa
H5L	36% of wild-type activity	Cannabis sativa
H5Q	22% of wild-type activity	Cannabis sativa
H5S	47% of wild-type activity	Cannabis sativa
H78N	0% of wild-type activity	Cannabis sativa
H78Q	0% of wild-type activity	Cannabis sativa
H78S	0% of wild-type activity	Cannabis sativa
I7F	64% of wild-type activity	Cannabis sativa
I7L	85% of wild-type activity	Cannabis sativa
V59M	65% of wild-type activity	Cannabis sativa
Y27F	162% of wild-type activity	Cannabis sativa
Y27L	48% of wild-type activity	Cannabis sativa
Y27M	30% of wild-type activity	Cannabis sativa
Y27W	37% of wild-type activity	Cannabis sativa
Y72F	0% of wild-type activity	Cannabis sativa

Organism

Organism	UniProt	Comment	Textmining
Cannabis sativa	I6WU39	-	-

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Release 2023.1 (January 2023)