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Literature summary for 4.4.1.25 extracted from

  • Denger, K.; Smits, T.H.; Cook, A.M.
    L-Cysteate sulpho-lyase, a widespread pyridoxal 5'-phosphate-coupled desulphonative enzyme purified from Silicibacter pomeroyi DSS-3(T) (2006), Biochem. J., 394, 657-664.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.25

General Stability

General Stability Organism
frozen samples lose about 90% of the activity on thawing Ruegeria pomeroyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.7
-
 L-cysteate pH 9.0, 30°C Ruegeria pomeroyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, SDS-PAGE Ruegeria pomeroyi
100000
-
 gel filtration Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteate + H2O Desulfovibrio sp. inducible enzyme pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O Bilophila wadsworthia inducible enzyme pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O Ruegeria pomeroyi inducible enzyme pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O Bilophila wadsworthia RZATAU inducible enzyme pyruvate + bisulfite + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Bilophila wadsworthia
-
 RZATAU
-
Bilophila wadsworthia RZATAU
-
 RZATAU
-
Desulfovibrio sp.
-
 strain RZACYSA and strain GRZCYSA
-
Ruegeria pomeroyi
-
 DSS-3
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruegeria pomeroyi

Storage Stability

Storage Stability Organism
4°C, 2 months without significant loss of activity Ruegeria pomeroyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteate + H2O
-
 Desulfovibrio sp. pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O
-
 Bilophila wadsworthia pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O
-
 Ruegeria pomeroyi pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O inducible enzyme Desulfovibrio sp. pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O inducible enzyme Bilophila wadsworthia pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O inducible enzyme Ruegeria pomeroyi pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O
-
 Bilophila wadsworthia RZATAU pyruvate + bisulfite + NH3
-
 ?
L-cysteate + H2O inducible enzyme Bilophila wadsworthia RZATAU pyruvate + bisulfite + NH3
-
 ?
additional information enzyme also catalyzes the D-cysteine desulphhydrase reaction Ruegeria pomeroyi ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Ruegeria pomeroyi

Synonyms

Synonyms Comment Organism
CuyA
-
 Ruegeria pomeroyi
L-cysteate sulpho-lyase (deaminating)
-
 Ruegeria pomeroyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9 enzyme in crude cell extract, 50 mM Tris buffer Ruegeria pomeroyi

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate tightly bound to the enzyme Ruegeria pomeroyi