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Literature summary for 4.4.1.24 extracted from

  • Rein, U.; Gueta, R.; Denger, K.; Ruff, J.; Hollemeyer, K.; Cook, A.M.
    Dissimilation of cysteate via 3-sulfolactate sulfo-lyase and a sulfate exporter in Paracoccus pantotrophus NKNCYSA (2005), Microbiology, 151, 737-747.
    View publication on PubMed
Search for more literature for 4.4.1.24

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is strongly inducible Paracoccus pantotrophus

Cloned(Commentary)

Cloned (Comment) Organism
gene suyAB, DNA and amino acid sequence determination and analysis Paracoccus pantotrophus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 70% inhibition at 1 mM Paracoccus pantotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Fe2+ at 2mM Paracoccus pantotrophus
Fe2+ required for activity Paracoccus pantotrophus
Mn2+ can substitute for Fe2+ at 2mM Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
 x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus
42000
-
 x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-sulfolactate Paracoccus pantotrophus desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview pyruvate + bisulfite
-
 ?
additional information Paracoccus pantotrophus activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus Q58Y44 strongly inducible enzyme, gene suyAB
-

Purification (Commentary)

Purification (Comment) Organism
from cysteate-grown cells by anion exchange chromatography and hydrophobic interaction chromatography, to near homogeneity Paracoccus pantotrophus

Reaction

Reaction Comment Organism Reaction ID
(2R)-3-sulfolactate = pyruvate + hydrogensulfite putative reaction mechanism Paracoccus pantotrophus
a

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:(R)-cysteate-grown cell i.e. 2-amino-3-sulfopropionate, is the sole carbon and energy source of the organism with either nitrate or molecular oxygen as terminal electron acceptor Paracoccus pantotrophus
-
culture condition:sulfolactate-grown cell
-
 Paracoccus pantotrophus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 purified enzyme Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-sulfolactate
-
 Paracoccus pantotrophus pyruvate + bisulfite
-
 ?
3-sulfolactate desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview Paracoccus pantotrophus pyruvate + bisulfite
-
 ?
additional information activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization Paracoccus pantotrophus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus

Synonyms

Synonyms Comment Organism
3-sulfolactate sulfo-lyase
-
 Paracoccus pantotrophus
More the enzyme belongs to the altronate dehydratase family Paracoccus pantotrophus
Suy
-
 Paracoccus pantotrophus